| De novo protein synthesis of syntaxin-1 and dynamin-1 in long-term memory formation requires CREB1 gene transcription in Lymnaea stagnalis. | |
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MedLine Citation:
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PMID: 20563839 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Consolidation of aversive operant conditioning into long-term memory (LTM) requires CREB-dependent de novo protein synthesis. The newly synthesized proteins are distributed to the synapses in neurons that are involved in memory formation and storage. Accumulating evidence indicates that the presynaptic release mechanisms also play a role in long-term synaptic plasticity. Our understanding of whether the presynaptic proteins undergo de novo synthesis during long-term memory formation is limited. In this study, we investigated the involvement of syntaxin-1, a presynaptic exocytotic protein, and dynamin-1, an endocytotic protein, in the formation of long-term memory. We took advantage of a well-established aversive operant conditioning model of aerial respiratory behavior in the fresh water pond snail Lymnaea stagnalis, and demonstrated that the LTM formation is associated with increased expression of syntaxin-1 and dynamin-1, coincident with elevated levels of CREB1. Partial knockdown of CREB1 gene by double stranded RNA inhibition (dsRNAi) prior to operant conditioning prevented snails from memory consolidation, and reduced the expression of syntaxin-1 and dynamin-1 at both mRNA and protein levels. These findings suggest that CREB1-mediated gene expression is required for the LTM-induced up-regulation of synaptic proteins, syntaxin-1 and dynamin-1, in L. stagnalis. Our study thus offers new insights into the molecular mechanisms that mediate CREB1-dependent long-term memory formation. |
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Authors:
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Cong-Hui Guo; Anthony Senzel; Kathy Li; Zhong-Ping Feng |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-06-20 |
Journal Detail:
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Title: Behavior genetics Volume: 40 ISSN: 1573-3297 ISO Abbreviation: Behav. Genet. Publication Date: 2010 Sep |
Date Detail:
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Created Date: 2010-08-13 Completed Date: 2011-01-03 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0251711 Medline TA: Behav Genet Country: United States |
Other Details:
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Languages: eng Pagination: 680-93 Citation Subset: IM |
Affiliation:
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Department of Physiology, Faculty of Medicine, University of Toronto, Toronto, ON M5S 1A8, Canada. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Conditioning, Classical / physiology Cyclic AMP Cyclic AMP Response Element-Binding Protein / genetics*, metabolism* Dynamin I / biosynthesis* Lymnaea Memory / physiology* Neurons / physiology Presynaptic Terminals / physiology* Qa-SNARE Proteins / biosynthesis* RNA Interference / physiology RNA, Messenger / metabolism Synapses / physiology |
| Grant Support | |
ID/Acronym/Agency:
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MOP62738//Canadian Institutes of Health Research |
| Chemical | |
Reg. No./Substance:
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0/Cyclic AMP Response Element-Binding Protein; 0/Qa-SNARE Proteins; 0/RNA, Messenger; 60-92-4/Cyclic AMP; EC 3.5.1.50/Dynamin I |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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