| Dark light, rod saturation, and the absolute and incremental sensitivity of mouse cone vision. | |
| | |
MedLine Citation:
|
PMID: 20844144 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Visual thresholds of mice for the detection of small, brief targets were measured with a novel behavioral methodology in the dark and in the presence of adapting lights spanning ∼8 log(10) units of intensity. To help dissect the contributions of rod and cone pathways, both wild-type mice and mice lacking rod (Gnat1(-/-)) or cone (Gnat2(cpfl3)) function were studied. Overall, the visual sensitivity of mice was found to be remarkably similar to that of the human peripheral retina. Rod absolute threshold corresponded to 12-15 isomerized pigment molecules (R*) in image fields of 800 to 3000 rods. Rod "dark light" (intrinsic retinal noise in darkness) corresponded to that estimated previously from single-cell recordings, 0.012 R* s(-1) rod(-1), indicating that spontaneous thermal isomerizations are responsible. Psychophysical rod saturation was measured for the first time in a nonhuman species and found to be very similar to that of the human rod monochromat. Cone threshold corresponded to ∼5 R* cone(-1) in an image field of 280 cones. Cone dark light was equivalent to ∼5000 R* s(-1) cone(-1), consistent with primate single-cell data but 100-fold higher than predicted by recent measurements of the rate of thermal isomerization of mouse cone opsins, indicating that nonopsin sources of noise determine cone threshold. The new, fully automated behavioral method is based on the ability of mice to learn to interrupt spontaneous wheel running on the presentation of a visual cue and provides an efficient and highly reliable means of examining visual function in naturally behaving normal and mutant mice. |
| | |
Authors:
|
Frank Naarendorp; Tricia M Esdaille; Serenity M Banden; John Andrews-Labenski; Owen P Gross; Edward N Pugh |
Related Documents
:
|
3350064 - Visual function and rhodopsin levels in humans with vitamin a deficiency. 3230484 - Saturation of rod-initiated signals in 2-month-old human infants. 18459824 - Strongly ph-buffered ringer's solution expands the receptive field size of horizontal c... 6980864 - Rapid dark adaptation of bullfrog rods is delayed by barium. 8445134 - Individual differences in the improvement in spectral shape discrimination due to incre... 2506144 - Localization and cure of epileptic foci with the use of meg measurements. |
Publication Detail:
|
Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't |
Journal Detail:
|
Title: The Journal of neuroscience : the official journal of the Society for Neuroscience Volume: 30 ISSN: 1529-2401 ISO Abbreviation: J. Neurosci. Publication Date: 2010 Sep |
Date Detail:
|
Created Date: 2010-09-16 Completed Date: 2010-10-25 Revised Date: 2011-09-22 |
Medline Journal Info:
|
Nlm Unique ID: 8102140 Medline TA: J Neurosci Country: United States |
Other Details:
|
Languages: eng Pagination: 12495-507 Citation Subset: IM |
Affiliation:
|
Department of Psychology, Northeastern University, Boston, Massachusetts 02115, USA. f.naarendorp@neu.edu |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Behavior, Animal / radiation effects Calibration Darkness* Female GTP-Binding Protein alpha Subunits / genetics, radiation effects Heterotrimeric GTP-Binding Proteins / genetics, radiation effects Male Mice Mice, Inbred C57BL Mice, Knockout Photic Stimulation Retinal Cone Photoreceptor Cells / physiology*, radiation effects Retinal Rod Photoreceptor Cells / physiology*, radiation effects Transducin / genetics, radiation effects Vision, Ocular / physiology*, radiation effects |
| Grant Support | |
ID/Acronym/Agency:
|
EY-02660/EY/NEI NIH HHS; R01 EY002660-32/EY/NEI NIH HHS |
| Chemical | |
Reg. No./Substance:
|
0/GTP-Binding Protein alpha Subunits; 0/Gnat1 protein, mouse; 0/Gnat2 protein, mouse; EC 3.6.1.-/Transducin; EC 3.6.5.1/Heterotrimeric GTP-Binding Proteins |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Neural correlates of auditory scene analysis based on inharmonicity in monkey primary auditory corte...
Next Document: A role for protein kinase C-dependent upregulation of adrenomedullin in the development of morphine ...