| Dark chocolate inhibits platelet isoprostanes via nox2 down-regulation in smokers. | |
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MedLine Citation:
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PMID: 22066819 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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Background: Dark chocolate is reported to decrease platelet activation but the underlying mechanism is still undefined. Dark chocolate is rich of polyphenols that could exert an antiplatelet action via inhibition of oxidative stress. Aim of the study was to assess if dark chocolate inhibits platelet reactive oxidant species (ROS) formation and platelet activation. Methods: Twenty healthy subjects (HS) and 20 smokers were randomly allocated to receive 40 g of dark (cocoa >85%) or milk chocolate (cocoa <35%) in a cross-over, single blind study. There was an interval of 7 days between the two phases of the study. At baseline and 2 hours after chocolate ingestion platelet recruitment (PR), platelet ROS, platelet isoprostane 8-ISO-prostaglandin F2α (8-iso-PGF2α), Thromboxane (TxA2) and platelet activation of NOX2, the catalytic sub-unit of NADPH oxidase, and serum epicatechin were measured. Results: Compared to HS, smokers showed enhanced PR, platelet formation of ROS and eicosanoids and NOX2 activation. After dark chocolate platelet ROS (-48%, p<0.001), 8-iso-PGF2α (-10%, p<0.001) and NOX2 activation (-22%, p<0.001) significantly decreased; dark chocolate did not affect platelet variables in HS. No effect of milk chocolate was detected in both groups. Serum epicatechin increased after dark chocolate in HS (from 0.454+/-0.3nM to 118.3+/-53.7nM) and smokers (from 0.5+/-0.28 nM to 120.9+/-54.2 nM). Platelet incubation with 0.1-10μM catechin significantly reduced PR, platelet 8-iso-PGF2α and ROS formation and NOX2 activation only in platelets from smokers. Conclusions: Dark chocolate inhibits platelet function by lowering oxidative stress only in smokers; this effect seems to be dependent on its polyphenolic content. |
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Authors:
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R Carnevale; L Loffredo; P Pignatelli; C Nocella; S Bartimoccia; S Di Santo; F Martino; E Catasca; L Perri; F Violi |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2011-11-8 |
Journal Detail:
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Title: Journal of thrombosis and haemostasis : JTH Volume: - ISSN: 1538-7836 ISO Abbreviation: - Publication Date: 2011 Nov |
Date Detail:
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Created Date: 2011-11-9 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101170508 Medline TA: J Thromb Haemost Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Copyright Information:
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Copyright © 2011 International Society on Thrombosis and Haemostasis. |
Affiliation:
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I Clinica Medica, Sapienza University, Rome Center of Clinic Lipid Research, Department of Pediatrics, and Division of Internal Medicine H., Rome, Italy. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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