Document Detail


Damage to ram spermatozoa by peroxidation of endogenous phospholipids.
MedLine Citation:
PMID:  881664     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We examined the damaging effects on spermatozoa of endogenous phospholipid peroxidation brought about by aerobic incubation at 37 degrees C in the presence of 0-5 mM-ascorbic acid and 0-5 mM-FeSO4. As well as becoming immotile, such peroxidized spermatozoa also lost, through leakage, certain intracellular enzymes into the surrounding medium, on a scale resembling that produced by cold shocking non-peroxidized spermatozoa. Morphological observations revealed that peroxidation damaged the plasma membrane, particularly in the region of the acrosome. Further experiments showed that lipid peroxidation irreversibly abolished the fructolytic and respiratory activity of spermatozoa. The susceptibility of spermatozoa to peroxidation was greater when the cells were damaged before incubation with ascorbic acid and FeSO4. To some extent, peroxidation could be prevented, but not reversed, by the addition to sperm suspensions of dialysed egg yolk or dialysed bull seminal plasma. However, dialysed seminal plasma from ram, stallion or man had no protective effect.
Authors:
R Jones; T Mann
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of reproduction and fertility     Volume:  50     ISSN:  0022-4251     ISO Abbreviation:  J. Reprod. Fertil.     Publication Date:  1977 Jul 
Date Detail:
Created Date:  1977-09-17     Completed Date:  1977-09-17     Revised Date:  2003-11-14    
Medline Journal Info:
Nlm Unique ID:  0376367     Medline TA:  J Reprod Fertil     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  261-8     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Fructose / biosynthesis
Male
Oxygen Consumption / drug effects
Peroxides / metabolism*
Phospholipids / metabolism*
Sheep
Sperm Motility / drug effects
Spermatozoa / metabolism*
Chemical
Reg. No./Substance:
0/Peroxides; 0/Phospholipids; 30237-26-4/Fructose

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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