Document Detail


Damage to oligodendrocytes in the striatum after MPTP neurotoxicity in mice.
MedLine Citation:
PMID:  17676428     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We investigated the alteration of oligodendrocytes in comparison with that of astrocytes and microglia in the mouse striatum after MPTP (1-methyl-4-phenyl-1,2,3,6-tetrahydropridine) treatment under the same conditions using Western blot analysis and Immunohistochemistry. In our Western blot analysis, four administrations of MPTP at 2-h intervals to mice produced the remarkable loss of TH (tyrosine hydroxylase) protein levels in the striatum after 3 and 7 days. In contrast, GFAP (glial fibrillary acidic protein) and Iba-1 protein in the striatum showed a significant increase of GFAP and Iba-1 protein levels 3 and 7 days after MPTP treatment. On the other hand, the levels of CNPase (2', 3'-cyclic nucleotide 3'-phosphodiesterase) protein were decreased significantly in the striatum 3 and 7 days after MPTP treatment. In our immunohistochemical study, a significant decrease in the area of expression of CNPase-positive profiles was observed in the striatum 3 and 7 days after MPTP treatment. These results demonstrate that oligodendrocytes in the striatum are damaged after MPTP treatment. Thus our present findings provide valuable information for the pathogenesis of Parkinson's disease.
Authors:
S Takagi; N Hayakawa; H Kimoto; H Kato; T Araki
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2007-08-06
Journal Detail:
Title:  Journal of neural transmission (Vienna, Austria : 1996)     Volume:  114     ISSN:  0300-9564     ISO Abbreviation:  J Neural Transm     Publication Date:  2007  
Date Detail:
Created Date:  2007-12-06     Completed Date:  2008-05-28     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9702341     Medline TA:  J Neural Transm     Country:  Austria    
Other Details:
Languages:  eng     Pagination:  1553-7     Citation Subset:  IM    
Affiliation:
Department of Neurobiology and Therapeutics, Graduate School and Faculty of Pharmaceutical Sciences, The University of Tokushima, Tokushima, Japan.
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MeSH Terms
Descriptor/Qualifier:
2',3'-Cyclic-Nucleotide Phosphodiesterases / drug effects,  metabolism
Animals
Astrocytes / drug effects,  pathology
Blotting, Western
Calcium-Binding Proteins / drug effects,  metabolism
Corpus Striatum / drug effects*,  metabolism,  pathology*
Glial Fibrillary Acidic Protein / drug effects,  metabolism
Immunohistochemistry
MPTP Poisoning / metabolism,  pathology*
Male
Mice
Mice, Inbred C57BL
Microglia / drug effects,  pathology
Oligodendroglia / drug effects*,  metabolism,  pathology*
Tyrosine 3-Monooxygenase / drug effects,  metabolism
Chemical
Reg. No./Substance:
0/Aif1 protein, mouse; 0/Calcium-Binding Proteins; 0/Glial Fibrillary Acidic Protein; EC 1.14.16.2/Tyrosine 3-Monooxygenase; EC 3.1.4.-/2',3'-Cyclic-Nucleotide Phosphodiesterases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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