Document Detail


Damage to ciliated epithelium in chronic rhinosinusitis: what is the role of bacterial biofilms?
MedLine Citation:
PMID:  19140536     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
OBJECTIVES: We assess the association between the presence of biofilms and cilial damage in patients with chronic rhinosinusitis (CRS), describe the microorganisms associated with samples that exhibited cilial loss and biofilms, and demonstrate the absence of ciliary injury and biofilms in similarly prepared "normal" controls. METHODS: We examined samples of ethmoid mucosa obtained from 24 patients who underwent functional endoscopic sinus surgery for CRS. Samples from a control group (20 healthy subjects) were also examined. The specimens were divided into 2 fragments; the first was processed for bacterial cultures, and the second was subjected to scanning electron microscopy. Statistical analysis was performed. RESULTS: All CRS samples had positive bacterial cultures. The scanning electron microscopy analysis showed bacterial biofilms in 10 of the 24 specimens. A marked destruction of the epithelium was observed in samples positive for biofilms (p < 0.001), and the presence of Haemophilus influenzae was associated with ciliary abnormalities (partial damage in 55.6% and absence of cilia in 50%; p = 0.041). CONCLUSIONS: The high percentage of biofilms in our specimens confirms the association between biofilms and CRS. Our data support the hypothesis that biofilm formation represents the latter phase of an inflammatory process that leads to complete epithelial destruction.
Authors:
Jacopo Galli; Lea Calò; Fausta Ardito; Micaela Imperiali; Ezio Bassotti; Giulio Cesare Passali; Giuseppe La Torre; Gaetano Paludetti; Giovanni Fadda
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  The Annals of otology, rhinology, and laryngology     Volume:  117     ISSN:  0003-4894     ISO Abbreviation:  Ann. Otol. Rhinol. Laryngol.     Publication Date:  2008 Dec 
Date Detail:
Created Date:  2009-01-14     Completed Date:  2009-02-10     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0407300     Medline TA:  Ann Otol Rhinol Laryngol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  902-8     Citation Subset:  AIM; IM    
Affiliation:
Institute of Otorhinolaryngology, Catholic University of the Sacred Heart, Rome, Italy.
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MeSH Terms
Descriptor/Qualifier:
Adult
Aged
Biofilms*
Case-Control Studies
Chronic Disease
Cilia / pathology*
Female
Humans
Male
Microscopy, Electron, Scanning
Middle Aged
Respiratory Mucosa / microbiology*,  pathology
Rhinitis / microbiology*,  pathology
Sinusitis / microbiology*,  pathology
Young Adult

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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