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Damage of staphylococcal cytoplasmic membrane by Quercus infectoria G. Olivier and its components.
MedLine Citation:
PMID:  21375550     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Aims:  In traditional Thai medicine, nutgall of Quercus infectoria G. Olivier is well- documented as an effective agent for wound and skin infections. The present study was aimed to establish modes of action of the ethanol extract of the plant as well as its main constituents to induce anti-methicillin-resistant Staphylococcus aureus (MRSA) activity. Methods and Results:  The minimal inhibitory concentration (MIC)/minimal bactericidal concentration (MBC) values of ethyl acetate I, ethyl acetate II, 95% ethanol, and 30% ethanol fractions against MRSA were 0.06/0.25, 0.13/0.25, 0.25/0.5, and 0.5/1.00 mg mL(-1) , respectively. Ellagic acid, gallic acid, syringic acid, and tannic acid as major components of Quercus infectoria nutgall extract were included in this study. Among these, gallic acid and tannic acid demonstrated good MIC/MBC values at 0.06/0.06 and 0.13/0.25 mg mL(-1) , respectively. A lysis experiment demonstrated that the ethanol extract, ethyl acetate fraction I, and all of the main components failed to lyse MRSA cells. Contrastly, both MRSA and S. aureus ATCC 25923 treated with the ethanol extract, ethyl acetate fraction I, gallic acid, and tannic acid displayed significant loss of tolerance to low osmotic pressure and high salt concentration. Conclusion:  The results documented effect of different fractions of Quercus infectoria and purified compounds on MRSA and S. aureus. In addition, the study demonstrated that treatment with Quercus infectoria extract and the purified compounds results in hypersensitivity to low and high osmotic pressure. Significance and Impact of the Study:  This study provides scientific information to support the traditional uses of the nutgall extract and suggesting its anti-MRSA mechanisms.
Authors:
S Chusri; S P Voravuthikunchai
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2011-3-4
Journal Detail:
Title:  Letters in applied microbiology     Volume:  -     ISSN:  1472-765X     ISO Abbreviation:  -     Publication Date:  2011 Mar 
Date Detail:
Created Date:  2011-3-7     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8510094     Medline TA:  Lett Appl Microbiol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2011 The Society for Applied Microbiology.
Affiliation:
Faculty of Traditional Thai Medicine, Prince of Songkla University, Hat Yai, Songkhla, Thailand Department of Microbiology and Natural Products Research Center, Faculty of Science, Prince of Songkla University, Hat Yai, Songkhla, Thailand.
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