| Dally and Notum regulate the switch between low and high level Hedgehog pathway signalling. | |
| | |
MedLine Citation:
|
PMID: 22872085 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
|
During development, secreted morphogens, such as Hedgehog (Hh), control cell fate and proliferation. Precise sensing of morphogen levels and dynamic cellular responses are required for morphogen-directed morphogenesis, yet the molecular mechanisms responsible are poorly understood. Several recent studies have suggested the involvement of a multi-protein Hh reception complex, and have hinted at an understated complexity in Hh sensing at the cell surface. We show here that the expression of the proteoglycan Dally in Hh-receiving cells in Drosophila is necessary for high but not low level pathway activity, independent of its requirement in Hh-producing cells. We demonstrate that Dally is necessary to sequester Hh at the cell surface and to promote Hh internalisation with its receptor. This internalisation depends on both the activity of the hydrolase Notum and the glycosyl-phosphatidyl-inositol (GPI) moiety of Dally, and indicates a departure from the role of the second glypican Dally-like in Hh signalling. Our data suggest that hydrolysis of the Dally-GPI by Notum provides a switch from low to high level signalling by promoting internalisation of the Hh-Patched ligand-receptor complex. |
| | |
Authors:
|
Katie L Ayers; Rana Mteirek; Alexandra Cervantes; Laurence Lavenant-Staccini; Pascal P Thérond; Armel Gallet |
Related Documents
:
|
21498475 - Co-inhibition of hsp70/hsp90 synergistically sensitizes nasopharyngeal carcinoma cells ... 22415765 - Cytotoxicity of hydroxyapatite nanoparticles is shape and cell dependent. 7698105 - Effects of asbestos and man-made vitreous fibers on cell division in cultured human mes... 22497875 - High-efficiency transduction of human monocyte-derived dendritic cells by capsid-modifi... 15684755 - Cell growth modulation of human cells irradiated in vitro with low-level laser therapy. 11029495 - Multiparameter flow cytometry for simultaneous assessment of p53 protein expression and... |
Publication Detail:
|
Type: Journal Article |
Journal Detail:
|
Title: Development (Cambridge, England) Volume: 139 ISSN: 1477-9129 ISO Abbreviation: Development Publication Date: 2012 Sep |
Date Detail:
|
Created Date: 2012-08-08 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 8701744 Medline TA: Development Country: England |
Other Details:
|
Languages: eng Pagination: 3168-79 Citation Subset: IM |
Affiliation:
|
Murdoch Childrens Research Institute, Royal Children's Hospital, Flemington Road Parkville Victoria 3052, Australia. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Anterior-posterior differences in HoxD chromatin topology in limb development.
Next Document: Hox genes regulate the onset of Tbx5 expression in the forelimb.