Document Detail

Dairy bacteria remove in vitro dietary lectins with toxic effects on colonic cells.
MedLine Citation:
PMID:  19191953     Owner:  NLM     Status:  In-Process    
AIMS: To assess in vitro the ability of some dairy bacteria to bind concanavalin A (Con A), peanut agglutinin (PNA) and jacalin (AIL), preventing their toxicity on mouse intestinal epithelial cells (IEC). METHODS AND RESULTS: Con A and AIL reduced significantly IEC viability in vitro, as determined by Trypan Blue dye exclusion or by propidium iodide/fluorescein diacetate/Hoescht staining. Different strains of dairy bacteria were able to remove lectins from the media. Two strains were subjected to treatments used to remove S-layer, cell wall proteins, polysaccharides and lectin-like adhesins. They were then assayed for the ability to bind dietary lectins and reduce toxicity against IEC and to adhere to IEC after interaction with lectins. Con A and AIL were removed by Propionibacterium acidipropionici and Propionibacterium freudenreichii by binding with specific sugar moieties on the bacterial surface. Removal of lectins by bacteria impaired IEC protection. Adhesion of P. acidipropionici to IEC was reduced but not abolished after binding Con A or AIL. CONCLUSIONS: Removal of Con A or AIL by dairy propionibacteria was effective to avoid the toxic effect against colonic cells in vitro. SIGNIFICANCE AND IMPACT OF THE STUDY: Consumption of foods containing these bacteria would be a tool to protect the intestinal epithelia.
G Zárate; A Perez Chaia
Related Documents :
2297543 - The role of carbohydrate moieties of cholecystokinin receptors in cholecystokinin octap...
976313 - Precipitin and mitogenic behavior of dimeric and tetrameric concanavalin a.
22990663 - Molecular dynamics and free energy studies on aurora kinase a and its mutant bound with...
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-01-21
Journal Detail:
Title:  Journal of applied microbiology     Volume:  106     ISSN:  1365-2672     ISO Abbreviation:  J. Appl. Microbiol.     Publication Date:  2009 Mar 
Date Detail:
Created Date:  2009-03-23     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9706280     Medline TA:  J Appl Microbiol     Country:  England    
Other Details:
Languages:  eng     Pagination:  1050-7     Citation Subset:  IM    
Centro de Referencias para Lactobacilos (CERELA)-CONICET, San Miguel de Tucumán, Argentina.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Molecular typing and identification of Bdellovibrio-and-like organisms isolated from seawater shrimp...
Next Document:  Atrazine degradation by stable mixed cultures enriched from agricultural soil and their characteriza...