| Daily variations in colchicine-induced apoptosis in duodenal crypts. | |
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MedLine Citation:
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PMID: 15865323 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Apoptotic cell death can be induced by several agents, among them colchicine, a microtubule disrupting-drug that affects continuously renewing cell populations, such as the intestinal crypt enterocytes. The objectives of this investigation were (1) to confirm in vivo colchicines-inductive effect and (2) to determine the existence of 24 h variations in the crypt enterocytes apoptotic indices. The study was done on C3H/S male adult mice housed under standardized conditions. Starting at midnight until the end of a circadian period, subgroups of mice were sacrificed after having been injected with colchicine or saline i.p. 4h beforehand. Duodenal samples were processed for hematoxylin-eosin staining and TUNEL technique. In order to score the number of apoptosis, the longitudinal sections of the crypts were divided into three regions comprised, respectively, of tiers 1-4, 5-12, and 13-20, proceeding from the bottom to the top of the crypt. Values of each lot were expressed as mean +/- SEM. A highly significant statistical difference in apoptotic indices was found for colchicine-treated animals. The 24 h curve for colchicine-induced apoptosis displayed qualitative and quantitative differences compared to other inducer agents. Highest apoptotic indices were found in the deepest crypt regions. Daily variations were observed in all the crypt sectors of the colchicine-treated animals and in tiers 5-12 of the saline controls. The present work demonstrates that the colchicine cytotoxicity due to its apoptotic-inducing effect depends on the dosing time during the 24 h in this mouse strain. |
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Authors:
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V González Norma; Amado F Badrán; Claudio G Barbeito |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Chronobiology international Volume: 22 ISSN: 0742-0528 ISO Abbreviation: Chronobiol. Int. Publication Date: 2005 |
Date Detail:
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Created Date: 2005-05-03 Completed Date: 2005-07-18 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8501362 Medline TA: Chronobiol Int Country: United States |
Other Details:
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Languages: eng Pagination: 79-88 Citation Subset: IM |
Affiliation:
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Laboratory of Histology and Embryology, School of Nature Sciences, National University of La Plata, Buenos Aires, Argentina. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Apoptosis* Circadian Rhythm* Colchicine / pharmacology* Duodenum / drug effects, metabolism* Gout Suppressants / pharmacology In Situ Nick-End Labeling Intestine, Small / drug effects Male Mice Mice, Inbred C3H Time Factors |
| Chemical | |
Reg. No./Substance:
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0/Gout Suppressants; 64-86-8/Colchicine |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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