Document Detail

Daily Rhythms of PERIOD protein in the eyestalk of the American lobster, Homarus americanus.
MedLine Citation:
PMID:  23487569     Owner:  NLM     Status:  Publisher    
The daily rhythm of PERIOD protein (PER) expression is an integral component of the circadian clock, which is found among a broad range of animal species including fruit flies, marine mollusks and even humans. The use of antibodies directed against PER has provided a helpful tool in the discovery of PER homologues and the labeling of putative pacemaker cells, especially in animals for which an annotated genome is not readily available. In this study, DrosophilaPER antibodies were used to probe for PER in the American lobster, Homarus americanus. This species exhibits robust endogenous circadian rhythms but the circadian clock has yet to be located or characterized. PER was detected in the eyestalks of the lobster but not in the brain. Furthermore, a significant effect of the LD cycle on daily PER abundance was identified, and PER was significantly more abundant at mid dark than in early light or mid light hours. Our results suggest that PER is a part of the molecular machinery of the circadian clock located in the eyestalk of the lobster.
Katharine R Grabek; Christopher C Chabot
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-10-2
Journal Detail:
Title:  Marine and freshwater behaviour and physiology     Volume:  45     ISSN:  1023-6244     ISO Abbreviation:  Mar Freshw Behav Physiol     Publication Date:  2012 Jan 
Date Detail:
Created Date:  2013-3-14     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101548682     Medline TA:  -     Country:  -    
Other Details:
Languages:  ENG     Pagination:  269-279     Citation Subset:  -    
Human Medical Genetics Program, Department of Cellular and Developmental Biology, University of Colorado School of Medicine, Aurora, CO 80045 USA.
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