Document Detail

DVC1 (C1orf124) recruits the p97 protein segregase to sites of DNA damage.
MedLine Citation:
PMID:  23042607     Owner:  NLM     Status:  MEDLINE    
Ubiquitin-binding domains (UBDs) are crucial for recruiting many proteins to sites of DNA damage. Here we characterize C1orf124 (Spartan; referred to as DVC1), which has an UBZ4-type UBD found predominantly in DNA repair proteins. DVC1 associates with DNA replication factories and localizes to sites of DNA damage in human cells, in a manner that requires the ability of the DVC1 UBZ domain to bind to ubiquitin polymers in vitro and a conserved PCNA-interacting motif. DVC1 interacts with the p97 protein 'segregase'. We show that DVC1 recruits p97 to sites of DNA damage, where we propose that p97 facilitates the extraction of the translesion synthesis (TLS) polymerase (Pol) η during DNA repair to prevent excessive TLS and limit the incidence of mutations induced by DNA damage. We introduce DVC1 as a regulator of cellular responses to DNA damage that prevents mutations when DNA damage occurs.
Emily J Davis; Christophe Lachaud; Paul Appleton; Thomas J Macartney; Inke Näthke; John Rouse
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2012-10-07
Journal Detail:
Title:  Nature structural & molecular biology     Volume:  19     ISSN:  1545-9985     ISO Abbreviation:  Nat. Struct. Mol. Biol.     Publication Date:  2012 Nov 
Date Detail:
Created Date:  2012-11-07     Completed Date:  2013-01-16     Revised Date:  2014-11-05    
Medline Journal Info:
Nlm Unique ID:  101186374     Medline TA:  Nat Struct Mol Biol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1093-100     Citation Subset:  IM    
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MeSH Terms
Adenosine Triphosphatases / metabolism*
Cell Cycle Proteins / metabolism*
DNA Damage / genetics,  physiology*
DNA-Binding Proteins / metabolism*
DNA-Directed DNA Polymerase / metabolism
Fluorescent Antibody Technique
Gene Knockdown Techniques
Proliferating Cell Nuclear Antigen / metabolism*
RNA, Small Interfering / genetics
Ubiquitin / metabolism*
Grant Support
11243//Cancer Research UK; MC_U127070192//Medical Research Council; //Cancer Research UK; //Medical Research Council
Reg. No./Substance:
0/Cell Cycle Proteins; 0/DNA-Binding Proteins; 0/Proliferating Cell Nuclear Antigen; 0/RNA, Small Interfering; 0/Spartan protein, human; 0/Ubiquitin; EC DNA Polymerase; EC protein; EC 3.6.1.-/Adenosine Triphosphatases; EC 3.6.1.-/CDC48 protein

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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