| DSC studies on interactions between low molecular mass peptide dendrimers and model lipid membranes. | |
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MedLine Citation:
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PMID: 16930888 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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It has recently been shown that a newly synthesized peptide dendrimers possess antimicrobial activity against Gram-positive (Staphylococcus aureus) and Gram-negative (Escherichia coli) bacteria as well as against fungal pathogens (Candida albicans) [Klajnert, B., Janiszewska, J., Urbanczyk-Lipkowska, Z., Bryszewska, M., Shcharbin, D., Labieniec, M., 2006. Biological properties of low molecular mass peptide dendrimers. Int. J. Pharm. 309, 208-217]. To extend our knowledge about their impact on biological systems, interactions between a group of low molecular mass lysine based dendrimers and model lipid bilayers were examined by differential scanning calorimetry (DSC). Conformational stability of dendrimers in 5-85 degrees C temperature range was confirmed by circular dichroism measurements (CD). The dendrimer structure has been shown to play an important role in interactions with the membranes. A two-step mechanism of dendrimer-bilayer interactions was proposed. The first step involves electrostatic attractions between dendrimers and polar lipid heads, while the second one is a result of hydrophobic interactions between acyl chains and arms of dendrimers. While one dendrimer did not interact with the membrane, another with long hydrophobic arms significantly perturbed the membrane. Nevertheless, for all tested dendrimers the main transition in DSC scans was retained that indicates that these compounds at the tested concentrations did not cause the loss of membrane integrity. |
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Authors:
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B Klajnert; J Janiszewska; Z Urbanczyk-Lipkowska; M Bryszewska; R M Epand |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2006-07-16 |
Journal Detail:
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Title: International journal of pharmaceutics Volume: 327 ISSN: 0378-5173 ISO Abbreviation: Int J Pharm Publication Date: 2006 Dec |
Date Detail:
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Created Date: 2006-11-07 Completed Date: 2007-03-13 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 7804127 Medline TA: Int J Pharm Country: Netherlands |
Other Details:
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Languages: eng Pagination: 145-52 Citation Subset: IM |
Affiliation:
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Department of General Biophysics, University of Lodz, 12/16 Banacha Street, 90-237 Lodz, Poland. aklajn@biol.uni.lodz.pl |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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1,2-Dipalmitoylphosphatidylcholine
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chemistry* Calorimetry, Differential Scanning Dendrimers / chemistry* Dimyristoylphosphatidylcholine / chemistry Lipid Bilayers / chemistry* Liposomes / chemistry Molecular Weight Peptides / chemistry* Phosphatidylglycerols / chemistry |
| Chemical | |
Reg. No./Substance:
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0/Dendrimers; 0/Lipid Bilayers; 0/Liposomes; 0/Peptides; 0/Phosphatidylglycerols; 13699-48-4/Dimyristoylphosphatidylcholine; 2644-64-6/1,2-Dipalmitoylphosphatidylcholine; 61361-72-6/dimyristoylphosphatidylglycerol |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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