Document Detail


DSC measurement of cell suspensions during successive freezing runs: implications for the mechanisms of intracellular ice formation.
MedLine Citation:
PMID:  7743814     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The formation of intracellular ice in biological cells during freezing is considered almost universally lethal and is the major contributor to cell damage at high cooling rates. Despite its importance, our understanding of the mechanisms of intracellular ice formation (IIF) is still incomplete. In this paper differential scanning calorimetry is used to study IIF in human lymphocytes in the presence of dimethyl sulfoxide (Me2SO). Under conditions where damage due to IIF on the initial cooling run is 40-60%, the samples are studied as a function of multiple successive cooling runs. This enables the study not only of the cell fraction which undergoes IIF, but also of the fraction which survives. The temperature at which IIF occurs and the fraction of cell volume which undergoes IIF are analyzed as functions of successive cooling runs. Taking advantage of the large number of cells present in the samples (ca. 10(6)), the effect of successive cooling runs on susceptibility to IIF is examined.
Authors:
G Bryant
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Publication Detail:
Type:  In Vitro; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Cryobiology     Volume:  32     ISSN:  0011-2240     ISO Abbreviation:  Cryobiology     Publication Date:  1995 Apr 
Date Detail:
Created Date:  1995-06-14     Completed Date:  1995-06-14     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  0006252     Medline TA:  Cryobiology     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  114-28     Citation Subset:  IM    
Affiliation:
Helmholtz-Institut für Biomedizinische Technik, RWTH Aachen, Federal Republic of Germany.
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MeSH Terms
Descriptor/Qualifier:
Calorimetry, Differential Scanning / methods*
Cell Death
Cell Size
Cryopreservation*
Dimethyl Sulfoxide
Humans
Ice*
Lymphocytes / metabolism,  ultrastructure
Osmotic Pressure
Temperature
Water / chemistry
Chemical
Reg. No./Substance:
0/Ice; 67-68-5/Dimethyl Sulfoxide; 7732-18-5/Water

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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