Document Detail

DRB2, DRB3 and DRB5 function in a non-canonical microRNA pathway in Arabidopsis thaliana.
MedLine Citation:
PMID:  22902697     Owner:  NLM     Status:  MEDLINE    
DOUBLE-STRANDED RNA BINDING (DRB) proteins have been functionally characterized in viruses, prokaryotes and eukaryotes and are involved in all aspects of RNA biology. Arabidopsis thaliana (Arabidopsis) encodes five closely related DRB proteins, DRB1 to DRB5. DRB1 and DRB4 are required by DICER-LIKE (DCL) proteins DCL1 and DCL4 to accurately and efficiently process structurally distinct double-stranded RNA (dsRNA) precursor substrates in the microRNA (miRNA) and trans-acting small-interfering RNA (tasiRNA) biogenesis pathways respectively. We recently reported that DRB2 is also involved in the biogenesis of specific miRNA subsets. ( 1) Furthermore, the severity of the developmental phenotype displayed by the drb235 triple mutant plant, compared with those expressed by either drb2, drb3 and drb5 single mutants, or double mutant combinations thereof, indicates that DRB3 and DRB5 function in the same non-canonical miRNA pathway as DRB2. Through the use of our artificial miRNA (amiRNA) plant expression vector, pBlueGreen ( 2) (,) ( 3) we demonstrate here that unlike DRB2, DRB3 and DRB5 are not involved in the dsRNA processing stages of the miRNA biogenesis pathway, but are required to mediate RNA silencing of target genes of DRB2-associated miRNAs.
Andrew L Eamens; Ki Wook Kim; Peter M Waterhouse
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2012-08-20
Journal Detail:
Title:  Plant signaling & behavior     Volume:  7     ISSN:  1559-2324     ISO Abbreviation:  Plant Signal Behav     Publication Date:  2012 Oct 
Date Detail:
Created Date:  2012-12-11     Completed Date:  2013-05-16     Revised Date:  2013-07-12    
Medline Journal Info:
Nlm Unique ID:  101291431     Medline TA:  Plant Signal Behav     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1224-9     Citation Subset:  IM    
School of Molecular Bioscience, University of Sydney, Sydney, NSW Australia.
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MeSH Terms
Arabidopsis / genetics*,  metabolism
Arabidopsis Proteins / metabolism*
Gene Knockout Techniques
Gene Silencing
MicroRNAs / genetics,  metabolism*
Models, Biological
Mutation / genetics
RNA-Binding Proteins / metabolism*
Reg. No./Substance:
0/Arabidopsis Proteins; 0/DRB2 protein, Arabidopsis; 0/DRB3 protein, Arabidopsis; 0/DRB5 protein, Arabidopsis; 0/MicroRNAs; 0/RNA-Binding Proteins

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