Document Detail

DR5 as a reporter system to study auxin response in Populus.
MedLine Citation:
PMID:  23283559     Owner:  NLM     Status:  Publisher    
KEY MESSAGE : Auxin responsive promoter DR5 reporter system is functional in Populus to monitor auxin response in tissues including leaves, roots, and stems. We described the behavior of the DR5::GUS reporter system in stably transformed Populus plants. We found several similarities with Arabidopsis, including sensitivity to native and synthetic auxins, rapid induction after treatment in a variety of tissues, and maximal responses in root tissues. There were also several important differences from Arabidopsis, including slower time to maximum response and lower induction amplitude. Young leaves and stem sections below the apex showed much higher DR5 activity than did older leaves and stems undergoing secondary growth. DR5 activity was highest in cortex, suggesting high levels of auxin concentration and/or sensitivity in this tissue. Our study shows that the DR5 reporter system is a sensitive and facile system for monitoring auxin responses and distribution at cellular resolution in poplar.
Yiru Chen; Yordan S Yordanov; Cathleen Ma; Steven Strauss; Victor B Busov
Related Documents :
24400489 - Large effects of consumer offense on ecosystem structure and function.
19184549 - Carboxylate platform: the mixalco process part 2: process economics.
24942979 - Plasticity in sunflower leaf and cell growth under high salinity.
19845039 - Optimised separation procedures for the simultaneous assay of three plant hormones in l...
24150569 - The impact of ionic mercury on antioxidant defenses in two mercury-sensitive anaerobic ...
23226379 - Sinorhizobium meliloti sigma factors rpoe1 and rpoe4 are activated in stationary phase ...
17468289 - Using the ralstonia solanacearum tat secretome to identify bacterial wilt virulence fac...
25261689 - Antimycobacterial activity of selected medicinal plants traditionally used in sudan to ...
23882849 - Preparation of zno nanowire using sludge from wastewater treatment.
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2013-1-3
Journal Detail:
Title:  Plant cell reports     Volume:  -     ISSN:  1432-203X     ISO Abbreviation:  Plant Cell Rep.     Publication Date:  2013 Jan 
Date Detail:
Created Date:  2013-1-3     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9880970     Medline TA:  Plant Cell Rep     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Michigan Technological University, School of Forest Research and Environmental Science, 1400 Townsend Drive, Houghton, MI, 49931, USA.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Screening promoters for Anthurium transformation using transient expression.
Next Document:  Galactoglucomannan oligosaccharides are assumed to affect tracheary element formation via interactio...