Document Detail

DPP4 gene DNA methylation in the omentum is associated with its gene expression and plasma lipid profile in severe obesity.
MedLine Citation:
PMID:  20847730     Owner:  NLM     Status:  MEDLINE    
Severely obese subjects with the metabolic syndrome (MS) have higher dipeptidyl peptidase-4 (DPP4) expression in their visceral adipose tissue (VAT) compared to obese individuals without MS. We tested the hypothesis that methylation level of CpG sites in the DPP4 promoter CpG island in VAT was genotype-dependent and associated with DPP4 mRNA abundance and MS-related phenotypes. The VAT DNA was extracted in 92 severely obese premenopausal women undergoing biliopancreatic derivation for the treatment of obesity. Women were nondiabetic and none of them used medication to treat MS features. Cytosine methylation rates (%) of 102 CpG sites in the DPP4 CpG island were assessed by pyrosequencing of sodium bisulfite-treated DNA. Methylation rates were >10% for CpG sites 94-102. Their mean methylation rate (%Meth(94-102)) was different between genotypes for DPP4 polymorphisms rs13015258 (P = 0.001), rs17848915 (P = 0.0004), and c.1926 G>A (P = 0.001). The %Meth(94-102) correlated negatively with DPP4 mRNA abundance (r = -0.25, P < 0.05) and positively with plasma high-density lipoprotein (HDL) cholesterol concentrations (r = 0.22, P < 0.05), whereas DPP4 mRNA abundance correlated positively with plasma total-/HDL-cholesterol ratio (r = 0.25; P < 0.05). In the VAT of nondiabetic severely obese women, genotype-dependent methylation levels of specific CpG sites in the DPP4 promoter CpG island were associated with DPP4 gene expression and variability in the plasma lipid profile. Higher DPP4 gene expression in VAT and its relationship with the plasma lipid profile may be explained by actually unknown DPP4 biological effect or, to another extent, may also be a marker of VAT inflammation known to be associated with metabolic disturbances.
Valérie Turcot; Luigi Bouchard; Geneviève Faucher; André Tchernof; Yves Deshaies; Louis Pérusse; Alexandre Bélisle; Simon Marceau; Simon Biron; Odette Lescelleur; Laurent Biertho; Marie-Claude Vohl
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-09-16
Journal Detail:
Title:  Obesity (Silver Spring, Md.)     Volume:  19     ISSN:  1930-739X     ISO Abbreviation:  Obesity (Silver Spring)     Publication Date:  2011 Feb 
Date Detail:
Created Date:  2011-01-31     Completed Date:  2011-04-29     Revised Date:  2012-08-13    
Medline Journal Info:
Nlm Unique ID:  101264860     Medline TA:  Obesity (Silver Spring)     Country:  United States    
Other Details:
Languages:  eng     Pagination:  388-95     Citation Subset:  IM    
Nutraceuticals and Functional Foods Institute, Quebec, Quebec, Canada.
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MeSH Terms
Biliopancreatic Diversion
DNA Methylation*
Dipeptidyl Peptidase 4 / genetics*
Gene Expression
Intra-Abdominal Fat / enzymology,  metabolism*
Lipids / blood
Metabolic Syndrome X / enzymology,  genetics*,  metabolism
Obesity, Morbid / enzymology,  genetics*,  metabolism,  surgery
Omentum / metabolism*
Polymorphism, Genetic
RNA, Messenger / genetics,  metabolism
Grant Support
MOP-164745//Canadian Institutes of Health Research
Reg. No./Substance:
0/Lipids; 0/RNA, Messenger; EC protein, human; EC Peptidase 4

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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