Document Detail


DOG1 and PKC-θ are useful in the diagnosis of KIT-negative gastrointestinal stromal tumors.
MedLine Citation:
PMID:  21358619     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Pathological diagnosis of gastrointestinal stromal tumors (GISTs) is based on histological findings and immunohistochemical demonstration of the KIT protein. KIT-negative GISTs account for ∼5% of cases and cause diagnostic difficulties. In the era of imatinib therapy, a correct diagnosis of GISTs is important for therapeutic reasons regardless of KIT expression. Recently, DOG1 has been introduced as an important diagnostic marker with high sensitivity and specificity. In this study, immunohistochemical staining for DOG1 and protein kinase C-θ (PKC-θ) in whole tissue sections, and mutation analyses for KIT and PDGFRA were performed in 26 KIT-negative GISTs. Tissue microarrays of 112 KIT-positive GISTs were used as controls. Overall, 25 KIT-negative GISTs were located in the stomach, and 1 in the rectum. The histological subtype was spindle in 12, epithelioid in 11, and mixed in 3 cases. The expression of DOG1 and PKC-θ was positive in 24 (92%) and in 25 cases (96%), respectively. All 26 KIT-negative GISTs expressed either DOG1 or PKC-θ, and 23 cases (89%) were positive for both makers. PKC-θ was positive in two cases (8%), which lacked both KIT and DOG1 expressions. Mutation analysis showed PDGFRA exon 18 mutation in 15 cases (58%) and KIT exon 11 mutation in 1 case (4%), whereas the remaining 10 cases (39%) were wild type for both KIT and PDGFRA. The expression of DOG1 and PKC-θ showed no significant difference in KIT-negative and KIT-positive GISTs (P=1.000 and P=0.167, respectively). Our findings suggest that both DOG1 and PKC-θ can be used in the diagnosis of KIT-negative GISTs and they show positive staining even in KIT-negative tumors, which are wild type for KIT and PDGFRA on mutation analysis.Modern Pathology advance online publication, 25 February 2011; doi:10.1038/modpathol.2011.11.
Authors:
Gu-Hyun Kang; Amitabh Srivastava; Young Eun Kim; Hee-Jung Park; Cheol Keun Park; Tae Sung Sohn; Sung Kim; Dae Young Kang; Kyoung-Mee Kim
Related Documents :
21237489 - Oncocytic adrenocortical neoplasms-a clinicopathologic study of 13 new cases emphasizin...
21196659 - A rare case of primary solid intrarectal dermoid.
7191579 - Subcutaneous phycomycosis caused by basidiobolus haptaosporus (drechsler, 1947).
21437439 - Fallopian tube origin of supposed ovarian high-grade serous carcinomas.
21316079 - Jazf1 and jjaz1 gene fusion in primary extrauterine endometrial stromal sarcoma.
17898519 - Exclusion and inclusion criteria for people with aphasia in studies of depression after...
7570939 - Transfusion-transmitted human parvovirus b19 infection in a thalassemic patient.
3262079 - Deficiency of protein c in patients with portal vein thrombosis.
2902529 - Evaluation of dead cases of ruptured intracranial aneurysms with special reference to a...
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2011-2-25
Journal Detail:
Title:  Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc     Volume:  -     ISSN:  1530-0285     ISO Abbreviation:  -     Publication Date:  2011 Feb 
Date Detail:
Created Date:  2011-3-1     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8806605     Medline TA:  Mod Pathol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Affiliation:
Department of Pathology, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, Korea.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Clinicopathological and molecular characterization of nine cases of columnar cell variant of papilla...
Next Document:  A primer to scaffolded DNA origami.