| The DOF transcription factor OBP1 is involved in cell cycle regulation in Arabidopsis thaliana. | |
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MedLine Citation:
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PMID: 18665917 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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In contrast to animal growth, plant growth is largely post-embryonic. Therefore plants have developed new mechanisms to precisely regulate cell proliferation by means of internal and external stimuli whilst the general core cell cycle machinery is conserved between eukaryotes. In this work we demonstrate a role for the Arabidopsis thaliana DNA-binding-with-one-finger (DOF) transcription factor OBP1 in the control of cell division upon developmental signalling. Inducible overexpression of OBP1 resulted in a significant overrepresentation of cell cycle genes among the upregulated transcripts. Direct targets of OBP1, as verified by chromatin immunoprecipitation, include at least the core cell cycle gene CYCD3;3 and the replication-specific transcription factor gene AtDOF2;3. Consistent with our molecular data, short-term activation of OBP1 in cell cultures affected cell cycle re-entry, shortening the duration of the G(1) phase and the overall length of the cell cycle, whilst constitutive overexpression of OBP1 in plants influenced cell size and cell number, leading to a dwarfish phenotype. Expression during embryogenesis, germination and lateral root initiation suggests an important role for OBP1 in cell cycle re-entry, operating as a transcriptional regulator of key cell cycle genes. Our findings provide significant input into our understanding of how cell cycle activity is incorporated into plant growth and development. |
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Authors:
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Aleksandra Skirycz; Amandine Radziejwoski; Wolfgang Busch; Matthew A Hannah; Joanna Czeszejko; Mirosław Kwaśniewski; Maria-Inès Zanor; Jan U Lohmann; Lieven De Veylder; Isabell Witt; Bernd Mueller-Roeber |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2008-09-04 |
Journal Detail:
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Title: The Plant journal : for cell and molecular biology Volume: 56 ISSN: 1365-313X ISO Abbreviation: Plant J. Publication Date: 2008 Dec |
Date Detail:
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Created Date: 2008-12-04 Completed Date: 2008-12-15 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9207397 Medline TA: Plant J Country: England |
Other Details:
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Languages: eng Pagination: 779-92 Citation Subset: IM |
Affiliation:
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Cooperative Research Group, Max-Planck Institute of Molecular Plant Physiology, Am Mühlenberg 1, 14476 Potsdam-Golm, Germany. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Arabidopsis
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cytology,
genetics*,
metabolism Arabidopsis Proteins / genetics, metabolism* Cell Cycle* Cell Cycle Proteins / metabolism Cell Proliferation Cells, Cultured Chromatin Immunoprecipitation DNA-Binding Proteins / genetics, metabolism* Electrophoretic Mobility Shift Assay Gene Expression Profiling Gene Expression Regulation, Developmental Gene Expression Regulation, Plant Genes, Plant Oligonucleotide Array Sequence Analysis RNA, Plant / genetics Transcription Factors / genetics, metabolism* |
| Chemical | |
Reg. No./Substance:
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0/Arabidopsis Proteins; 0/Cell Cycle Proteins; 0/DNA-Binding Proteins; 0/OBP protein, Arabidopsis; 0/RNA, Plant; 0/Transcription Factors |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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