| DNase I sensitivity of the alpha 2(I) collagen gene: correlation with its expression but not with its methylation pattern. | |
| | |
| Jump to Full Text | |
MedLine Citation:
|
PMID: 6328424 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
The chromatin structure of the chick alpha 2(I) collagen gene was probed with DNase I. Because our previous work strongly suggested that the 5' end of this gene is not methylated whereas the rest of the gene is methylated whether or not the gene is expressed, we compared the relative DNase I sensitivity of the methylated and unmethylated segments. Both regions demonstrate similar relative DNase I sensitivities within a given tissue. In chromatin of chick embryo fibroblasts, we find a DNase I hypersensitive site which maps between 100 and 300 bp preceding the start of transcription. This site is not found in brain chromatin but is present in chick embryo fibroblasts transformed by Rous Sarcoma virus although the rate of transcription of the alpha 2(I) collagen gene is greatly reduced in these cells. Hence, the mechanism responsible for the large decrease in alpha 2(I) collagen gene expression in RSV transformed cells is different from the mechanism that is responsible for the presence of a DNase I hypersensitive site in the promoter. Furthermore, changes in the DNase I sensitivity of the chromatin of the alpha 2(I) collagen promoter occur without changes in the methylation pattern of the gene. |
| | |
Authors:
|
C McKeon; I Pastan; B de Crombrugghe |
Related Documents
:
|
16193234 - Patterns and localization of gene expression during intramembranous bone regeneration i... 15210554 - Potential role of sox9 in patterning tracheal cartilage ring formation in an embryonic ... 19272164 - Global comparative transcriptome analysis of cartilage formation in vivo. 16133144 - Maturational differences in superficial and deep zone articular chondrocytes. 16193234 - Patterns and localization of gene expression during intramembranous bone regeneration i... 9742094 - Mutations in rna polymerase ii and elongation factor sii severely reduce mrna levels in... |
Publication Detail:
|
Type: Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
|
Title: Nucleic acids research Volume: 12 ISSN: 0305-1048 ISO Abbreviation: Nucleic Acids Res. Publication Date: 1984 Apr |
Date Detail:
|
Created Date: 1984-06-22 Completed Date: 1984-06-22 Revised Date: 2009-11-18 |
Medline Journal Info:
|
Nlm Unique ID: 0411011 Medline TA: Nucleic Acids Res Country: ENGLAND |
Other Details:
|
Languages: eng Pagination: 3491-502 Citation Subset: IM |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Avian Sarcoma Viruses / genetics Cell Nucleus / metabolism Cell Transformation, Viral Cells, Cultured Chick Embryo Chromatin / physiology Collagen / genetics* DNA Restriction Enzymes Deoxyribonuclease I Endodeoxyribonucleases / metabolism* Fibroblasts / metabolism Genes* Methylation Nucleic Acid Hybridization Substrate Specificity Transcription, Genetic |
| Grant Support | |
ID/Acronym/Agency:
|
GM07727/GM/NIGMS NIH HHS |
| Chemical | |
Reg. No./Substance:
|
0/Chromatin; 9007-34-5/Collagen; EC 3.1.-/Endodeoxyribonucleases; EC 3.1.21.-/DNA Restriction Enzymes; EC 3.1.21.1/Deoxyribonuclease I |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
| Full Text | |
|
Journal Information Journal ID (nlm-ta): Nucleic Acids Res ISSN: 0305-1048 ISSN: 1362-4962 |
Article Information Download PDF  Print publication date: Day: 25 Month: 4 Year: 1984 Volume: 12 Issue: 8 First Page: 3491 Last Page: 3502 ID: 318764 PubMed Id: 6328424 |
| DNase I sensitivity of the alpha 2(I) collagen gene: correlation with its expression but not with its methylation pattern. | |
| C McKeon | |
| I Pastan | |
| B de Crombrugghe | |
Article Categories:
|
|
Previous Document: Structural organization of the 3' half of the rat thyroglobulin gene.
Next Document: Methylation dependent expression of the mom gene of bacteriophage Mu: deletions downstream from the ...