Document Detail

DNase I footprinting of the human interleukin-5 gene promoter.
MedLine Citation:
PMID:  10651947     Owner:  NLM     Status:  MEDLINE    
A characteristic feature of allergic asthma is the overexpression of the T helper type 2 (Th2) cytokines interleukin-4 (IL-4), IL-5 and IL-13 by T lymphocytes. Of these cytokines, IL-5 is critical for the growth, survival and recruitment of eosinophils which are thought to be responsible for the tissue damage observed in asthmatic airways. The expression of human IL-5 is primarily regulated at the transcriptional level; however, little is known about the mechanisms that control its transcription. Using nuclear extracts from allergen-specific human T-cell clones we have performed DNase I footprinting of the human IL-5 promoter in order to establish sites occupied by transcription factors. We show footprints covering the conserved lymphokine element 0 ¿(CLE0) -60 to -44 base pairs (bp) and GATA (-73 to -62 bp) elements, which have previously been identified to be important in the regulation of the murine IL-5 promoter. We also describe a footprint covering a considerably extended Octamer binding site (-249 to -217 bp), which encompasses two hitherto unidentified CCAAT/enhancer binding protein consensus binding sites. We have also identified a previously unknown Ets binding site (-274 to -264 bp). These novel data on the regions of the human IL-5 promoter that are bound by transcription factors should allow dissection of the regulatory mechanisms involved in the transcription of IL-5 in the T-helper lymphocytes of asthmatics.
D J Cousins; D Richards; D M Kemeny; S Romagnani; T H Lee; D Z Staynov
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Immunology     Volume:  99     ISSN:  0019-2805     ISO Abbreviation:  Immunology     Publication Date:  2000 Jan 
Date Detail:
Created Date:  2000-02-10     Completed Date:  2000-02-10     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  0374672     Medline TA:  Immunology     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  101-8     Citation Subset:  IM    
Department of Respiratory Medicine and Allergy, King's College London, London, UK.
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MeSH Terms
Asthma / immunology*
Binding Sites
Clone Cells
DNA Footprinting
Deoxyribonuclease I
Gene Expression Regulation*
Interleukin-5 / genetics*
Promoter Regions, Genetic*
Reverse Transcriptase Polymerase Chain Reaction
T-Lymphocytes / immunology*
Transcription Factors
Reg. No./Substance:
0/Interleukin-5; 0/Transcription Factors; EC I

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