Document Detail


DNMT3L is a novel marker and is essential for the growth of human embryonal carcinoma.
MedLine Citation:
PMID:  20460473     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
PURPOSE: Testicular germ cell tumors (TGCT) have a unique epigenetic profile distinct from that of other types of cancer. Elucidation of these properties has a potential to identify novel markers for TGCTs. EXPERIMENTAL DESIGN: We conducted comprehensive analysis of DNA methyltransferase (DNMT) gene expression in TGCTs. Based on the expression profiles of DNMT genes in TGCTs, we generated a rabbit polyclonal anti-human DNMT3L antibody. We then studied the role of DNMT3L in TGCTs by the treatment of two embryonal carcinoma (EC) cell lines with a small interfering RNA system. Finally, we evaluated the immunohistochemical detection of DNMT3L in TGCT tissues. We also compared the patterns of DNMT3L immunohistochemistry with those of CD30 and SOX2. RESULTS: Among the DNMT genes, we found that mRNA for DNMT3L was specifically expressed in TGCTs, but neither in normal testicular tissues nor in cancer cells of somatic tissue origin. DNMT3L protein was strongly expressed in two EC cell lines, but not in the cell lines of somatic tissue origin. Transfection of small interfering RNA for DNMT3L significantly reduced DNMT3L expression and resulted in growth suppression and apoptosis in EC cells. Immunohistochemical analysis showed that DNMT3L protein was present only in EC cells, but not in the other types of TGCT components and cancer cells of somatic tissue origin. DNMT3L staining was more prominent and specific than CD30 or SOX2 staining for detecting EC cells. CONCLUSION: DNMT3L is a novel marker and is essential for the growth of human embryonal carcinoma.
Authors:
Kahori Minami; Tokuhiro Chano; Takahiro Kawakami; Hiroshi Ushida; Ryoji Kushima; Hidetoshi Okabe; Yusaku Okada; Keisei Okamoto
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-05-11
Journal Detail:
Title:  Clinical cancer research : an official journal of the American Association for Cancer Research     Volume:  16     ISSN:  1078-0432     ISO Abbreviation:  Clin. Cancer Res.     Publication Date:  2010 May 
Date Detail:
Created Date:  2010-05-14     Completed Date:  2010-08-16     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9502500     Medline TA:  Clin Cancer Res     Country:  United States    
Other Details:
Languages:  eng     Pagination:  2751-9     Citation Subset:  IM    
Copyright Information:
Copyright (c) 2010 AACR.
Affiliation:
Department of Clinical Laboratory Medicine, Shiga University of Medical Science, Shiga, Japan.
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MeSH Terms
Descriptor/Qualifier:
Blotting, Western
Carcinoma, Embryonal / genetics*,  metabolism
Cell Separation
DNA (Cytosine-5-)-Methyltransferase / biosynthesis*,  genetics
Flow Cytometry
Gene Expression
Gene Expression Profiling
Humans
Immunohistochemistry
Male
Microscopy, Fluorescence
RNA, Messenger / analysis
RNA, Small Interfering
Reverse Transcriptase Polymerase Chain Reaction
Testicular Neoplasms / genetics*,  metabolism
Transfection
Tumor Markers, Biological / genetics*
Chemical
Reg. No./Substance:
0/RNA, Messenger; 0/RNA, Small Interfering; 0/Tumor Markers, Biological; EC 2.1.1.-/DNMT3L protein, human; EC 2.1.1.37/DNA (Cytosine-5-)-Methyltransferase

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