| DNA-synthesis inhibition and repair DNA-synthesis in CHO Ade-C cells: an alternative approach to genotoxicity testing. | |
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MedLine Citation:
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PMID: 9473778 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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We describe an alternative assay to determine genotoxicity. Its main feature is that it combines two measures in a single experiment; the inhibition of replicative DNA synthesis together with the stimulation of DNA repair. We show that, in tests of four different genotoxic agents, the assay gives results that are entirely consistent with what is known about the mode of action of these agents. In addition, we have demonstrated that chemical carcinogens requiring metabolic activation can be examined using a standard procedure of incubation with a microsomal activating fraction. We consider the combined assay for DNA synthesis inhibition and repair synthesis to be a useful way for the rapid prescreening of chemicals suspected of genotoxic activity on the level of mammalian cells. |
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Authors:
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D Slamenová; A Collins; L Wsólová; E Papsová; A Gábelová; M Dusinská |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Neoplasma Volume: 44 ISSN: 0028-2685 ISO Abbreviation: Neoplasma Publication Date: 1997 |
Date Detail:
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Created Date: 1998-03-02 Completed Date: 1998-03-02 Revised Date: 2007-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0377266 Medline TA: Neoplasma Country: CZECH REPUBLIC |
Other Details:
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Languages: eng Pagination: 247-52 Citation Subset: IM |
Affiliation:
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Cancer Research Institute of Slovak Academy of Sciences, Bratislava, Slovakia. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Benzopyrenes / pharmacology* Biotransformation CHO Cells Cricetinae DNA Repair / drug effects* DNA Replication / drug effects* Evaluation Studies as Topic Hypoxanthine / metabolism Methylnitrosourea / pharmacology Mutagenicity Tests Mutagens / pharmacology* Nitroquinolines / pharmacology |
| Chemical | |
Reg. No./Substance:
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0/Benzopyrenes; 0/Mutagens; 0/Nitroquinolines; 68-94-0/Hypoxanthine; 684-93-5/Methylnitrosourea |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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