Document Detail


DNA-synthesis inhibition and repair DNA-synthesis in CHO Ade-C cells: an alternative approach to genotoxicity testing.
MedLine Citation:
PMID:  9473778     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We describe an alternative assay to determine genotoxicity. Its main feature is that it combines two measures in a single experiment; the inhibition of replicative DNA synthesis together with the stimulation of DNA repair. We show that, in tests of four different genotoxic agents, the assay gives results that are entirely consistent with what is known about the mode of action of these agents. In addition, we have demonstrated that chemical carcinogens requiring metabolic activation can be examined using a standard procedure of incubation with a microsomal activating fraction. We consider the combined assay for DNA synthesis inhibition and repair synthesis to be a useful way for the rapid prescreening of chemicals suspected of genotoxic activity on the level of mammalian cells.
Authors:
D Slamenová; A Collins; L Wsólová; E Papsová; A Gábelová; M Dusinská
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Neoplasma     Volume:  44     ISSN:  0028-2685     ISO Abbreviation:  Neoplasma     Publication Date:  1997  
Date Detail:
Created Date:  1998-03-02     Completed Date:  1998-03-02     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  0377266     Medline TA:  Neoplasma     Country:  CZECH REPUBLIC    
Other Details:
Languages:  eng     Pagination:  247-52     Citation Subset:  IM    
Affiliation:
Cancer Research Institute of Slovak Academy of Sciences, Bratislava, Slovakia.
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MeSH Terms
Descriptor/Qualifier:
Animals
Benzopyrenes / pharmacology*
Biotransformation
CHO Cells
Cricetinae
DNA Repair / drug effects*
DNA Replication / drug effects*
Evaluation Studies as Topic
Hypoxanthine / metabolism
Methylnitrosourea / pharmacology
Mutagenicity Tests
Mutagens / pharmacology*
Nitroquinolines / pharmacology
Chemical
Reg. No./Substance:
0/Benzopyrenes; 0/Mutagens; 0/Nitroquinolines; 68-94-0/Hypoxanthine; 684-93-5/Methylnitrosourea

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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