Document Detail


DNA synthesis in circulating erythroblasts of anemic duck. Isolation and properties of nuclear and cytoplasmic-nonmitochondrial DNA.
MedLine Citation:
PMID:  1204648     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
In the circulating blood of anemic ducks, 5% of all erythroid cells synthesize DNA. Immature erythroblasts, at all stages of differentiation, synthesize DNA although to a varying degree, while reticulocytes and erythrocytes do not. In the erythroid cell population labeled in vitro 2 h with 32Pi, half of the labeled DNA sediments as small-molecular-weight molecules, suggesting that these molecules fail to integrate into the high-molecular-weight components. Labeled DNA is found in the cytoplasmic postmitochondrial fractions and it is in a form of deoxyribonucleoproteins which cosediment with ribosomes as well as subribosomal particles in sucrose gradients. However, fixation with HCHO and centrifugation to equilibrium in CsCl gradient of these particles shows that the deoxyribonucleoprotein bands at the density different than the ribosomes and, thus, not physically linked to them. In EDTA-dissociated ribosomes, the deoxyribonucleoprotein particles cosediment with ribosomes as well as subribosomal particles in sucorse gradients. However, fixation with HCHO and centrifugation to equilibrium in CsCl gradient of these particles shows that the deoxyribonucleoprotein bands at the density different than the ribosomes and, thus, not physically linked to them. In EDTA-dissociated ribosomes, the deoxyribonucleoprotein particles cosdeiment with ribosomal subunits in such a way that the larger the particle, the larger the molecular weight of the DNA cosedimenting with it. The specific radioactivity of the cytoplasmic ribosome-derived and postribosomal-particle-derived DNAs and the small molecular-weight nuclear DNA is similar and 10-20-fold higher than that of the bulk nuclear DNA. The former three DNA species sediment between 4-14 S. It is concluded that the cytoplasmic nonmitochondrial DNA species are of the nuclear origin. Less than 0.5% of the total cellular nonmitochondrial DNA can be purified from the nucleus and the cytoplasm as fast-labeled small-molecular-weight components. All of the cellular nonmitochondrial DNA species band at the same mean buoyand density in Cs2SO4/urea gradients. All behave as native structures in hydroxyapatite and contain less than 5% of their length as single-stranded regions.
Authors:
S P Modak; D Commelin; L Grosset; M T Imaizumi; M Monnat; K Scherrer
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  European journal of biochemistry / FEBS     Volume:  60     ISSN:  0014-2956     ISO Abbreviation:  Eur. J. Biochem.     Publication Date:  1975 Dec 
Date Detail:
Created Date:  1976-03-30     Completed Date:  1976-03-30     Revised Date:  2007-07-23    
Medline Journal Info:
Nlm Unique ID:  0107600     Medline TA:  Eur J Biochem     Country:  GERMANY, WEST    
Other Details:
Languages:  eng     Pagination:  407-21     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Anemia / blood*
Animals
Cell Nucleus / metabolism*
Centrifugation, Density Gradient
Cytoplasm / metabolism
DNA / blood*
DNA Replication*
Ducks
Embryo, Nonmammalian
Endonucleases
Erythrocytes / metabolism*
Molecular Weight
Polyribosomes / metabolism
Reticulocytes / metabolism
Subcellular Fractions / metabolism
Chemical
Reg. No./Substance:
9007-49-2/DNA; EC 3.1.-/Endonucleases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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