Document Detail


DNA repair capacity measured by high throughput alkaline comet assays in EBV-transformed cell lines and peripheral blood cells from cancer patients and healthy volunteers.
MedLine Citation:
PMID:  16260175     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We collected peripheral blood (PB) from 556 patients with various types of cancer who had undergone radiotherapy and from 81 healthy volunteers. We exposed whole PB and Epstein-Barr virus-transformed lymphoblastoid cell lines (EBLs) derived from the PB mononucleocytes to X-irradiation (5 Gy). Using the alkaline comet assay, we measured the immediate DNA damage and, at 15 min, the % residual damage. In PB, the immediate damage was similar in patients and healthy volunteers while the % residual damage (mean+/-S.D.) was significantly higher in patients with breast (54.3+/-A23.9), cervical (54.7+/-A23.9), head/neck (56.8+/-A24.4), lung (60.1+/-23.5), or esophageal cancers (59.5+/-A33.7) than in healthy donors (42.9+/-19.6) (P<0.05). We did not observe such differences in the EBV-transformed cell lines. Thus, radiation sensitivity of fresh PB cells measured by the alkaline comet assay was related to cancer status.
Authors:
Mayumi Iwakawa; Miyako Goto; Shuhei Noda; Masashi Sagara; Shigeru Yamada; Naoto Yamamoto; Yoshihiro Kawakami; Yoshifumi Matsui; Yukimasa Miyazawa; Hideya Yamazaki; Hiroshi Tsuji; Tatsuya Ohno; Junetsu Mizoe; Hirohiko Tsujii; Takashi Imai
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Publication Detail:
Type:  Comparative Study; Journal Article     Date:  2005-11-02
Journal Detail:
Title:  Mutation research     Volume:  588     ISSN:  0027-5107     ISO Abbreviation:  Mutat. Res.     Publication Date:  2005 Dec 
Date Detail:
Created Date:  2005-11-18     Completed Date:  2006-01-18     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0400763     Medline TA:  Mutat Res     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  1-6     Citation Subset:  IM    
Affiliation:
RadGenomics Project, Frontier Research Center, National Institute of Radiological Sciences, 4-9-1, Anagawa, Inage-ku, Chiba 263-8555, Japan.
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MeSH Terms
Descriptor/Qualifier:
Blood Donors
Cell Line, Transformed / radiation effects
Comet Assay / methods*
DNA Damage / radiation effects*
DNA Repair*
Female
Herpesvirus 4, Human
Humans
Leukocytes, Mononuclear / radiation effects*
Neoplasms / blood,  genetics*,  radiotherapy
Radiation Tolerance / genetics*

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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