Document Detail

DNA rearrangement and restriction fragment length polymorphism within the first BCR intron in Philadelphia-positive acute leukemia.
MedLine Citation:
PMID:  2569268     Owner:  NLM     Status:  MEDLINE    
In some patients with Philadelphia (Ph)-chromosome positive acute lymphoblastic leukemias, breakpoints on chromosome 22 are reported to occur within the first BCR intron. To analyze the breakpoints in chromosome 22 of Ph-positive acute leukemia patients without rearrangement of the 5.8 kb bcr, we cloned the 3' part of the first BCR intron using a synthetic DNA probe. During the course of study, we mapped the region of the deletion/insertion of 1 kb that causes a restriction fragment length polymorphism (RFLP) and found a racial difference in the frequencies of the alleles giving rise to this RFLP. Analyses of the patients' samples indicated that breakpoints were located within the 8.5 kb EcoRl fragment of the first BCR intron in two of five Ph-positive acute leukemia patients. The data, together with the previous reports, indicate that breakpoints within this approximately 50 kb intron are widely scattered, in contrast to those confined within the 5.8 kb bcr in chronic myelogenous leukemias.
Y Nakamura; O Miura; Y Sugahara; N Maseki; Y Kaneko; N Aoki
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  American journal of hematology     Volume:  32     ISSN:  0361-8609     ISO Abbreviation:  Am. J. Hematol.     Publication Date:  1989 Sep 
Date Detail:
Created Date:  1989-09-01     Completed Date:  1989-09-01     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7610369     Medline TA:  Am J Hematol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  24-9     Citation Subset:  IM    
First Department of Medicine, Tokyo Medical and Dental University, Japan.
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MeSH Terms
Acute Disease
Blotting, Southern
Cloning, Molecular
DNA / genetics*
Gene Rearrangement*
Leukemia / genetics*
Philadelphia Chromosome
Polymorphism, Genetic*
Polymorphism, Restriction Fragment Length*
Reg. No./Substance:

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