Document Detail


DNA-protein cross-links in erythrocytes of freshwater fish exposed to hexavalent chromium or divalent nickel.
MedLine Citation:
PMID:  18506500     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
DNA-protein cross-links (DPXs) in fish erythrocytes represent a potential biomarker for exposure to metal cations, such as hexavalent chromium (Cr[VI]) and divalent nickel (Ni[II]). Species-specific sensitivities to DPX formation were studied by coexposure of juvenile specimens of rainbow trout, hybrid bluegill, and channel catfish to waterborne metals, such as Cr(VI) and Ni(II). In a species comparison, 4 days of exposure to 2 ppm Cr(VI) induced highest DPXs in bluegill erythrocytes, followed by trout and catfish, at 186%, 97%, and 48% above controls, respectively. A similar pattern of species sensitivity was observed following co-exposure of the fish to 15 ppm Ni(II) for 4 days, with 237%, 124%, and 82% increased DPXs above control bluegill, trout, and catfish, respectively. Biological stability of Cr(VI)-induced DPXs was demonstrated in Cr(VI)-exposed bluegill, as DPX levels remained elevated for up to 20 days after discontinuation of exposure. Similar results were found following exposure of catfish to Ni(II), with detectable DPXs found 10 days after acute exposure. In both bluegill and catfish, a continued increase in DPX formation in erythrocytes was seen for 5-10 days after Cr(VI) was removed from tank water, suggesting that residual Cr(VI) may be involved in DPX formation following acute exposure of fish.
Authors:
Jim R Kuykendall; Kyle L Miller; Kristen M Mellinger; Andrew J Cain; Michael W Perry; Michael Bradley; Eric J Jarvi; Dennis J Paustenbach
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2008-05-28
Journal Detail:
Title:  Archives of environmental contamination and toxicology     Volume:  56     ISSN:  1432-0703     ISO Abbreviation:  Arch. Environ. Contam. Toxicol.     Publication Date:  2009 Feb 
Date Detail:
Created Date:  2009-01-12     Completed Date:  2009-03-27     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0357245     Medline TA:  Arch Environ Contam Toxicol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  260-7     Citation Subset:  IM    
Affiliation:
Raabe College of Pharmacy, Ohio Northern University, 525 South Main Street, Ada, OH 45810, USA. Jim.Toxigen@gmail.com
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MeSH Terms
Descriptor/Qualifier:
Animals
Biological Markers / blood
Blood Proteins / metabolism*
DNA / blood,  metabolism*
DNA Damage*
Erythrocytes / drug effects*,  metabolism
Fishes / blood*
Fresh Water
Nickel / toxicity*
Potassium Dichromate / toxicity*
Water Pollutants, Chemical / toxicity*
Chemical
Reg. No./Substance:
0/Biological Markers; 0/Blood Proteins; 0/Water Pollutants, Chemical; 7440-02-0/Nickel; 7718-54-9/nickel chloride; 7778-50-9/Potassium Dichromate; 9007-49-2/DNA

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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