Document Detail

DNA microarray technology used for studying foodborne pathogens and microbial habitats: minireview.
MedLine Citation:
PMID:  12180686     Owner:  NLM     Status:  MEDLINE    
Microarray analysis is an emerging technology that has the potential to become a leading trend in bacterial identification in food and feed improvement. The technology uses fluorescent-labeled probes amplified from bacterial samples that are then hybridized to thousands of DNA sequences immobilized on chemically modified glass slides. The whole gene or open reading frame(s) is represented by a polymerase chain reaction fragment of double-strand DNA, approximately 1000 base pair (bp) or 20-70 bp single-strand oligonucleotides. The technology can be used to identity bacteria and to study gene expression in complex microbial populations, such as those found in food and gastrointestinal tracts. Data generated by microarray analysis can be potentially used to improve the safety of our food supply as well as ensure the efficiency of animal feed conversion to human food, e.g., in meat and milk production by ruminants. This minireview addresses the use of microarray technology in bacterial identification and gene expression in different microbial systems and in habitats containing mixed populations of bacteria.
Sufian F Al-Khaldi; Scott A Martin; Avraham Rasooly; Jeff D Evans
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Publication Detail:
Type:  Journal Article; Review    
Journal Detail:
Title:  Journal of AOAC International     Volume:  85     ISSN:  1060-3271     ISO Abbreviation:  J AOAC Int     Publication Date:    2002 Jul-Aug
Date Detail:
Created Date:  2002-08-15     Completed Date:  2003-04-07     Revised Date:  2008-03-17    
Medline Journal Info:
Nlm Unique ID:  9215446     Medline TA:  J AOAC Int     Country:  United States    
Other Details:
Languages:  eng     Pagination:  906-10     Citation Subset:  IM    
U.S. Food and Drug Administration, CFSAN, Division of Microbiological Studies, College Park, MD 20740-3855, USA.
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MeSH Terms
Bacteria / genetics,  isolation & purification,  pathogenicity
Bacteriological Techniques
DNA, Bacterial / genetics,  isolation & purification
Food Microbiology*
Genes, Bacterial
Oligonucleotide Array Sequence Analysis*
Rumen / microbiology
Reg. No./Substance:
0/DNA, Bacterial

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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