Document Detail

DNA microarray analysis of the expression profiles of luteinized granulosa cells as a function of ovarian reserve.
MedLine Citation:
PMID:  12057731     Owner:  NLM     Status:  MEDLINE    
OBJECTIVE: To examine the expression profiles of luteinized granulosa cells isolated from women with normal or diminished ovarian reserve undergoing in vitro fertilization. DESIGN: Expression profiling by complementary DNA microarray analysis. SETTING: Women undergoing in vitro fertilization-embryo transfer in a university-based fertility clinic. PATIENT(S): Eighteen women with normal or decreased ovarian reserve. INTERVENTION(S): All patients were given gonadotropin stimulation in preparation for IVF with granulosa cells isolated at the time of follicular aspiration. MAIN OUTCOME MEASURE(S): Expression profiles of luteinized granulosa cells isolated from each woman were determined by using DNA microarray analysis. RESULT(S): Changes in patterns of gene expression in granulosa cells were observed between women with normal and diminished ovarian reserve. These genes included several anonymous expressed sequence tags and also expressed sequence tags that correspond to known genes. CONCLUSION(S): Expression profiling of granulosa cells by DNA microarray may yield signature patterns that reflect the status of ovarian reserve and the competence of granulosa cells.
Khew-Voon Chin; David B Seifer; Bo Feng; Yong Lin; Wei-Chung Shih
Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Fertility and sterility     Volume:  77     ISSN:  0015-0282     ISO Abbreviation:  Fertil. Steril.     Publication Date:  2002 Jun 
Date Detail:
Created Date:  2002-06-11     Completed Date:  2002-07-03     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0372772     Medline TA:  Fertil Steril     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1214-8     Citation Subset:  IM    
The Cancer Institute of New Jersey, UMDNJ-Robert Wood Johnson Medical School, New Brunswick, New Jersey 08901, USA.
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MeSH Terms
Corpus Luteum / physiopathology*
DNA, Complementary / genetics
Gene Expression Profiling*
Granulosa Cells / physiology*
Infertility, Female / genetics*,  physiopathology*
Oligonucleotide Array Sequence Analysis
Ovary / physiopathology*
Grant Support
Reg. No./Substance:
0/DNA, Complementary

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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