Document Detail


DNA methylation: a secondary event in globin gene switching?
MedLine Citation:
PMID:  2458298     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Changes in gamma-globin gene methylation accompany the fetal to adult globin switch in man. Using somatic cell hybrids made by fusing mouse erythroleukemia and human fetal erythroid cells, we asked whether methylation is a cause or a consequence of gamma-gene inactivation during development. These hybrids initially express human gamma-globin but switch with time in culture to adult globin gene production. In hybrids before the switch, the gamma-genes are unmethylated. After completion of the switch, the hybrids contain methylated gamma-globin genes. However, during the time that the gamma----beta switch is occurring, hybrids are found that no longer express gamma-globin, yet still possess unmethylated gamma-globin genes. This suggests that methylation is not a primary event in gamma-gene inactivation during human development.
Authors:
T Enver; J W Zhang; T Papayannopoulou; G Stamatoyannopoulos
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Genes & development     Volume:  2     ISSN:  0890-9369     ISO Abbreviation:  Genes Dev.     Publication Date:  1988 Jun 
Date Detail:
Created Date:  1988-10-24     Completed Date:  1988-10-24     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  8711660     Medline TA:  Genes Dev     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  698-706     Citation Subset:  IM    
Affiliation:
Division of Medical Genetics, University of Washington, Seattle 98195.
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MeSH Terms
Descriptor/Qualifier:
Adult
Cell Line
DNA / genetics*
Erythroblasts / metabolism
Fetal Hemoglobin / genetics*
Fetus
Genes*
Globins / genetics*
Hemoglobin A / genetics*
Humans
Hybrid Cells / metabolism
Methylation
Phenotype
Grant Support
ID/Acronym/Agency:
5R01 AM30852/AM/NIADDK NIH HHS; 5R01 HL2089/HL/NHLBI NIH HHS
Chemical
Reg. No./Substance:
9004-22-2/Globins; 9007-49-2/DNA; 9034-51-9/Hemoglobin A; 9034-63-3/Fetal Hemoglobin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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