Document Detail


DNA methylation profiling at imprinted loci after periconceptional micronutrient supplementation in humans: results of a pilot randomized controlled trial.
MedLine Citation:
PMID:  22267336     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Intrauterine exposures mediated by maternal diet may affect risk of cardiovascular disease, obesity, and type 2 diabetes. Recent evidence, primarily from animal studies and observational data in humans, suggests that the epigenome can be altered by maternal diet during the periconceptional period and that these programming events may underlie later disease risk. A randomized controlled trial of periconceptional micronutrient supplementation in The Gambia, where seasonal nutritional variations affect fetal growth and postnatal outcomes, provided a unique opportunity to test this hypothesis. Specifically, we targeted imprinted genes, which play important roles in allocation of maternal resources while being epigenetically regulated. DNA methylation at 12 differentially methylated regions (DMRs) was analyzed in cord blood samples from 58 offspring of women participating in a double-blind randomized-controlled trial of pre- and periconceptional micronutrient supplementation (including folate, zinc, and vitamins A, B, C, and D). We observed sex-specific effects of micronutrient supplementation, reducing methylation levels at two of the DMRs analyzed, IGF2R in girls and GTL2-2 in boys. This pilot study is the first to analyze DNA methylation in the context of a randomized controlled trial, and it provides suggestive evidence that periconceptional maternal nutrition alters offspring methylation at imprinted loci.
Authors:
Wendy N Cooper; Batbayar Khulan; Stephen Owens; Cathy E Elks; Veronica Seidel; Andrew M Prentice; Gusztav Belteki; Ken K Ong; Nabeel A Affara; Miguel Constância; David B Dunger
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Publication Detail:
Type:  Journal Article; Randomized Controlled Trial; Research Support, Non-U.S. Gov't     Date:  2012-01-20
Journal Detail:
Title:  FASEB journal : official publication of the Federation of American Societies for Experimental Biology     Volume:  26     ISSN:  1530-6860     ISO Abbreviation:  FASEB J.     Publication Date:  2012 May 
Date Detail:
Created Date:  2012-05-01     Completed Date:  2012-06-29     Revised Date:  2014-02-20    
Medline Journal Info:
Nlm Unique ID:  8804484     Medline TA:  FASEB J     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1782-90     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Adolescent
Adult
DNA Methylation*
Female
Gene Expression Profiling*
Humans
Micronutrients / administration & dosage*
Middle Aged
Pilot Projects
Placebos
Preconception Care
Young Adult
Grant Support
ID/Acronym/Agency:
MC_U106179472//Medical Research Council; MC_U123292699//Medical Research Council; MC_U123292700//Medical Research Council; //Biotechnology and Biological Sciences Research Council
Chemical
Reg. No./Substance:
0/Micronutrients; 0/Placebos

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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