Document Detail

DNA methylation changes in cells regrowing after fractioned ionizing radiation.
MedLine Citation:
PMID:  21704414     Owner:  NLM     Status:  Publisher    
BACKGROUND AND PURPOSE: Repeated exposure to ionizing radiation (IR) can result in adaptive reactions. While DNA methylation changes in adaption to repeated stress exposure are established for a variety of drugs, their role in fractioned ionizing radiation is largely unknown. MATERIAL AND METHODS: MCF7 breast cancer cells were treated 5 times a week with IR in fractions of 2Gy, resulting in total doses of 10 and 20Gy. Cells were harvested 48 and 72h after the last irradiation, as well as after a recovery period of at least 14 d. To identify genes differentially methylated in irradiated versus non-irradiated cells, we used methyl-CpG immunoprecipitation (MCIp) followed by global methylation profiling on CpG island microarrays. RESULTS: MCIp profiling revealed methylation changes in several CpG islands 48h after FIR with 10 and 20Gy. Cells receiving a total dose of 10Gy started regrowing after 14 d and exhibited similar radioresistance as mock-treated cells. Differential methylation of the CpG units associated with FOXC1 (p<0.001) and TRAPPC9 (p<0.001) could be confirmed by time-of-flight mass spectrometry (Sequenom). CONCLUSIONS: In summary, these data indicate that regrowth of MCF7 cells after 10Gy FIR is associated with locus-specific alterations in DNA methylation.
Christine Kuhmann; Dieter Weichenhan; Michael Rehli; Christoph Plass; Peter Schmezer; Odilia Popanda
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2011-6-23
Journal Detail:
Title:  Radiotherapy and oncology : journal of the European Society for Therapeutic Radiology and Oncology     Volume:  -     ISSN:  1879-0887     ISO Abbreviation:  -     Publication Date:  2011 Jun 
Date Detail:
Created Date:  2011-6-27     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8407192     Medline TA:  Radiother Oncol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2011 Elsevier Ireland Ltd. All rights reserved.
Division of Epigenomics and Cancer Risk Factors, German Cancer Research Center (DKFZ), Heidelberg, Germany.
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