Document Detail


DNA-mediated gene transfer into epidermal cells using electroporation.
MedLine Citation:
PMID:  3459459     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A reliable method for the introduction of foreign DNA into epidermal cells is described. Electroporation of murine BALB/c MK-1 epidermal cells with pSV2-CAT resulted in the transient expression of chloramphenicol-acetyltransferase (0.03 to 0.05 nmoles acetylchloramphenicol per mg protein per min) in the transfected cells. Transfection of MK-1 cells with pSV2-neo led to the appearance of approximately eight G418 resistant clones per 10(-6) cells per microgram of plasmid DNA. Distinct patterns of integration of SV2-neo were detected in three different resistant clones.
Authors:
M Reiss; M M Jastreboff; J R Bertino; R Narayanan
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Biochemical and biophysical research communications     Volume:  137     ISSN:  0006-291X     ISO Abbreviation:  Biochem. Biophys. Res. Commun.     Publication Date:  1986 May 
Date Detail:
Created Date:  1986-07-18     Completed Date:  1986-07-18     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0372516     Medline TA:  Biochem Biophys Res Commun     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  244-9     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Acetyltransferases / genetics
Animals
Cell Line
Cell Membrane Permeability
Chloramphenicol O-Acetyltransferase
Cloning, Molecular
Electricity
Epidermis / cytology,  physiology*
Genetic Engineering / methods*
Mice
Time Factors
Transfection*
Grant Support
ID/Acronym/Agency:
CA28852/CA/NCI NIH HHS
Chemical
Reg. No./Substance:
EC 2.3.1.-/Acetyltransferases; EC 2.3.1.28/Chloramphenicol O-Acetyltransferase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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