Document Detail


DNA measurements by single nuclei flow cytometry in human actinic skin lesions.
MedLine Citation:
PMID:  7310165     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A basal cell carcinoma, a Bowenoid carcinoma and clinically normal nonexposed skin from a 76-yr-old male with numerous epidermal tumors on sun-damaged skin were investigated by DNA flow cytometry and the results were compared with data obtained from normal skin of a control group. From each type of skin lesion 3 different biopsies were examined. Each of the DNA frequency distribution histograms showed bimodal configuration revealing no more than one DNA stem line, which was diploid in the unaffected skin and the Bowen carcinoma and hyperdiploid in the basal cell carcinoma. The 3 different types of skin could be clearly separated by means of at least 1 of 3 parameters: S-fraction, G2 + M fraction or S/G2 + M index. The S/G2 + M index came out as the most sensitive discriminator for the tumors. The S-fraction level was higher in the clinically normal skin of the investigated male than in the control group and further high in the tumors.
Authors:
G Frentz; U Møller
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The Journal of investigative dermatology     Volume:  77     ISSN:  0022-202X     ISO Abbreviation:  J. Invest. Dermatol.     Publication Date:  1981 Dec 
Date Detail:
Created Date:  1982-02-12     Completed Date:  1982-02-12     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0426720     Medline TA:  J Invest Dermatol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  431-3     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Aged
Carcinoma, Basal Cell / pathology
Carcinoma, Squamous Cell / pathology
Cell Cycle
Cell Nucleus / analysis*
DNA / analysis*
Flow Cytometry / methods*
Humans
Male
Skin / analysis*
Skin Neoplasms / analysis,  pathology*
Chemical
Reg. No./Substance:
9007-49-2/DNA

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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