| DNA loop formation between Nag repressor molecules bound to its two operator sites is necessary for repression of the nag regulon of Escherichia coli in vivo. | |
| | |
MedLine Citation:
|
PMID: 7934873 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Binding sites for the Nag repressor overlap the transcription start sites of the divergent nagE and nagB genes, such that the centres of the sites are separated by nine turns of the B-DNA helix. Mutations which prevent repressor binding to either site or alter the phasing of the binding sites result in simultaneous derepression of both genes. An additional mutation which restores the phasing of the two sites permits repression. These observations show that repression is the result of co-operative binding of the repressor to its two sites, resulting in the formation of a loop of DNA. |
| | |
Authors:
|
J Plumbridge; A Kolb |
Related Documents
:
|
19336003 - Moderately thermostable phage phi11 cro repressor has novel dna-binding capacity and ph... 17426033 - Glyoxylate and pyruvate are antagonistic effectors of the escherichia coli iclr transcr... 22828513 - Biophysical studies with aicd-47 reveals unique binding behavior characteristic of an u... |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
|
Title: Molecular microbiology Volume: 10 ISSN: 0950-382X ISO Abbreviation: Mol. Microbiol. Publication Date: 1993 Dec |
Date Detail:
|
Created Date: 1994-11-23 Completed Date: 1994-11-23 Revised Date: 2008-11-21 |
Medline Journal Info:
|
Nlm Unique ID: 8712028 Medline TA: Mol Microbiol Country: ENGLAND |
Other Details:
|
Languages: eng Pagination: 973-81 Citation Subset: IM |
Affiliation:
|
Institut de Biologie Physico-chimique (URA1139), Paris, France. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Bacterial Proteins
/
genetics,
metabolism* Base Sequence Binding Sites / genetics Carrier Proteins Cyclic AMP / metabolism Cyclic AMP Receptor Protein / metabolism DNA, Bacterial / chemistry, genetics, metabolism* Escherichia coli / genetics*, metabolism* Escherichia coli Proteins* Molecular Sequence Data Mutation Nucleic Acid Conformation Operator Regions, Genetic Recombinant Fusion Proteins / genetics, metabolism Regulon Repressor Proteins / genetics, metabolism* Transcription Factors* |
| Chemical | |
Reg. No./Substance:
|
0/Bacterial Proteins; 0/Carrier Proteins; 0/Cyclic AMP Receptor Protein; 0/DNA, Bacterial; 0/Escherichia coli Proteins; 0/Recombinant Fusion Proteins; 0/Repressor Proteins; 0/Transcription Factors; 0/nagC protein, E coli; 60-92-4/Cyclic AMP |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: The appearance of the UmuD'C protein complex in Escherichia coli switches repair from homologous rec...
Next Document: Construction and use of integrative vectors to express foreign genes in mycobacteria.