Document Detail


DNA identification of fetal cells isolated from cervical mucus: potential for early non-invasive prenatal diagnosis.
MedLine Citation:
PMID:  15842283     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
OBJECTIVES: To develop a reliable method to isolate fetal cells for genetic diagnosis. DESIGN: Aspiration of cervical mucus from pregnant women in the first trimester. SETTING: Pregnant women were recruited before an elective termination of pregnancy. POPULATION: Sixty pregnant women (7-10 weeks of gestation). METHODS: Fetal cells were isolated from aspirated cervical mucus of pregnant women using a combination of enzymatic digestion, fluorescent immunohistochemistry, micromanipulation and single-cell DNA allelic profiling. MAIN OUTCOME MEASURES: The isolation and identification of fetal cells. RESULTS: The transformation of the tenacious cervical mucus into a single-cell suspension enabled the isolation and identification of fetal cells by fluorescent immunohistochemistry. Confirmation of fetal origin was accomplished by single-cell DNA allelic profiling alongside known maternal cells. CONCLUSIONS: This novel non-invasive method is rapid and efficient with results attainable within 24 hours as early as seven weeks of gestation. The technique would offer earlier reassurance and the option of first trimester therapeutic abortions to both high and low risk pregnant women.
Authors:
Mandy G Katz-Jaffe; Debbie Mantzaris; David S Cram
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  BJOG : an international journal of obstetrics and gynaecology     Volume:  112     ISSN:  1470-0328     ISO Abbreviation:  BJOG     Publication Date:  2005 May 
Date Detail:
Created Date:  2005-04-21     Completed Date:  2005-06-14     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  100935741     Medline TA:  BJOG     Country:  England    
Other Details:
Languages:  eng     Pagination:  595-600     Citation Subset:  AIM; IM    
Affiliation:
Monash Institute of Reproduction and Development, Monash University and Monash IVF, Melbourne, Australia.
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MeSH Terms
Descriptor/Qualifier:
Cervix Mucus / cytology*
Chromosome Disorders / diagnosis
Cytogenetic Analysis / methods
DNA / analysis
DNA Fingerprinting / methods
Female
Fetus / cytology*
Fluorescent Antibody Technique
Genetic Testing / methods
Humans
Pregnancy
Pregnancy Trimester, First
Prenatal Diagnosis / methods*
Trophoblasts / cytology*
Chemical
Reg. No./Substance:
9007-49-2/DNA
Comments/Corrections
Comment In:
BJOG. 2005 Dec;112(12):1666-7; author reply 1667   [PMID:  16305574 ]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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