Document Detail

DNA-hydrolyzing activity of IgG antibodies from the sera of patients with tick-borne encephalitis.
MedLine Citation:
PMID:  20138955     Owner:  NLM     Status:  MEDLINE    
DNase autoantibodies (Abzs) can be found in the blood of patients with several autoimmune diseases, while the blood of healthy donors or patients with diseases with an insignificant disturbance of the immune status does not contain DNase Abzs. Here we present the first analysis of the DNase Abzs activity in the patients with tick-borne encephalitis (TBE). Several strict criteria have been applied to show that the DNase activity is an intrinsic property of IgGs from the sera of TBE patients but not from healthy donors. The relative activity of IgGs has been shown to vary extensively from patient to patient, but most of the preparations (91%) had detectable levels of the DNase activity. Polyclonal DNase IgGs were not active in the presence of EDTA or after a dialysis against EDTA, but could be activated by several externally added metal ions, with the level of activity decreasing in the order Mn(2+) + Ca(2+) > or = Mn(2+)+ Mg(2+) > or = Mn(2+) > or = Mg(2+) + Ca(2+) > or = Co(2+) > or = Mg(2+) > Ca(2+), while K(+), Na(+), Ni(2+), Zn(2+), and Cu(2+) did not stimulate DNA hydrolysis. Affinity chromatography on DNA-cellulose separated the DNase IgGs into many subfractions with various affinities for DNA and very different levels of the relative activity. Possible reasons for catalytic diversity of polyclonal human Abzs are discussed.
Taisiya A Parkhomenko; Valentina N Buneva; Ol'ga B Tyshkevich; Igor I Generalov; Boris M Doronin; Georgy A Nevinsky
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-02-06
Journal Detail:
Title:  Biochimie     Volume:  92     ISSN:  1638-6183     ISO Abbreviation:  Biochimie     Publication Date:  2010 May 
Date Detail:
Created Date:  2010-04-07     Completed Date:  2010-07-29     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  1264604     Medline TA:  Biochimie     Country:  France    
Other Details:
Languages:  eng     Pagination:  545-54     Citation Subset:  IM    
Copyright Information:
Copyright 2010 Elsevier Masson SAS. All rights reserved.
Institute of Chemical Biology and Fundamental Medicine, Russian Academy of Sciences, Siberian Division, Novosibirsk 630090, Russia.
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MeSH Terms
Chromatography, Liquid
DNA / metabolism*
Electrophoresis, Polyacrylamide Gel
Encephalitis, Tick-Borne / metabolism*
Enzyme-Linked Immunosorbent Assay
Immunoglobulin G / blood*,  isolation & purification
Metals / metabolism
Reg. No./Substance:
0/Immunoglobulin G; 0/Metals; 9007-49-2/DNA

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