| DNA hybridization assay for detection of Salmonella in foods: collaborative study. | |
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MedLine Citation:
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PMID: 3610967 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A collaborative study was performed in 11 laboratories to validate a DNA hybridization (DNAH) procedure for detection of Salmonella in foods. The DNAH procedure was compared to the standard culture method for detection of Salmonella in 6 foods: ground pepper, soy flour, dry whole egg, milk chocolate, nonfat dry milk, and raw deboned turkey. With the exception of turkey which was naturally contaminated, uninoculated and inoculated samples of each food group were analyzed. Results for the DNAH method were significantly better than for the standard culture method at the 5% probability level for the detection of Salmonella in turkey. There was no significant difference between the methods for the other 5 foods. The method has been adopted official first action. |
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Authors:
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R S Flowers; M J Klatt; M A Mozola; M S Curiale; D A Gabis; J H Silliker |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Journal - Association of Official Analytical Chemists Volume: 70 ISSN: 0004-5756 ISO Abbreviation: J Assoc Off Anal Chem Publication Date: 1987 May-Jun |
Date Detail:
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Created Date: 1987-08-28 Completed Date: 1987-08-28 Revised Date: 2003-11-14 |
Medline Journal Info:
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Nlm Unique ID: 7505559 Medline TA: J Assoc Off Anal Chem Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 521-9 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cacao / analysis DNA, Bacterial / genetics Dairy Products / analysis Eggs / analysis Filtration Food Microbiology* Indicators and Reagents Meat / analysis Nucleic Acid Hybridization* Salmonella / genetics, isolation & purification* Soybeans / analysis Turkeys |
| Chemical | |
Reg. No./Substance:
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0/DNA, Bacterial; 0/Indicators and Reagents |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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