Document Detail


DNA fingerprinting intron-sizing method to accomplish a specific, rapid, and sensitive identification of carotenogenic dunaliella species.
MedLine Citation:
PMID:  22623309     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
Dunaliella salina has become the most important microorganism for the production of β-carotene around the world. Natural carotenoids are a source of active metabolites utilized in different areas of food nutrition and pharmaceuticals, both in humans and also in animals. Identification of Dunaliella species from natural environments or certified culture collections is not precise and it is time consuming. However, accurate identification is extremely important because a slight difference in Dunaliella species generates great differences in carotenoids production. Here, we describe an intron-sizing method to make a rapid and precise identification for each of the most important carotenogenic species, showing that each hyperproducer species has an exclusive 18S rDNA fingerprint profile.
Authors:
Jorge Olmos-Soto; J Paniagua-Michel; Rosalía Contreras; Leonel Ochoa
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Methods in molecular biology (Clifton, N.J.)     Volume:  892     ISSN:  1940-6029     ISO Abbreviation:  Methods Mol. Biol.     Publication Date:  2012  
Date Detail:
Created Date:  2012-05-24     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9214969     Medline TA:  Methods Mol Biol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  269-81     Citation Subset:  IM    
Affiliation:
Department of Marine Biotechnology, Center for Scientific Research and Higher Education of Ensenada (CICESE), Ensenada, BC, Mexico, jolmos@cicese.mx.
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