Document Detail


DNA fingerprinting by means of a nonradioactive probe of sulfonated DNA.
MedLine Citation:
PMID:  2810896     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A DNA fingerprinting technique that uses a cold sulfonated DNA probe is described. DNA was extracted from blood samples of a family, who were subjected to DNA fingerprinting. The restriction fragments that were hybridized with the probe were colorimetrically detected by an enzyme immunoassay. For DNA detection and labeling a commercially available kit was employed. The sensitivity of this fingerprint detection method has been proven comparable to detection by the radioactive method, and hence is easier to use in conventional laboratories.
Authors:
E Naito; K Dewa; H Yamanouchi; K Mitani; R Kominami
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Nihon hōigaku zasshi = The Japanese journal of legal medicine     Volume:  43     ISSN:  0047-1887     ISO Abbreviation:  Nihon Hoigaku Zasshi     Publication Date:  1989 Jun 
Date Detail:
Created Date:  1989-12-15     Completed Date:  1989-12-15     Revised Date:  2006-09-04    
Medline Journal Info:
Nlm Unique ID:  0413715     Medline TA:  Nihon Hoigaku Zasshi     Country:  JAPAN    
Other Details:
Languages:  eng     Pagination:  243-5     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
DNA / genetics*
DNA Probes
Humans
Nucleotide Mapping
Chemical
Reg. No./Substance:
0/DNA Probes; 9007-49-2/DNA

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