Document Detail

DNA ethylation in target and non-target organs of hamsters and rats treated with diethylnitrosamine.
MedLine Citation:
PMID:  3971349     Owner:  NLM     Status:  MEDLINE    
Kinetics of ethylation of target and non-target organ DNA in vivo by diethylnitrosamine (DEN) was compared in rats and Syrian golden hamsters, since published reports indicate a single dose of DEN induces both kidney and liver tumors in rats and almost exclusively respiratory tract tumors in hamsters. Following treatment with 200 mg DEN/kg, 7-ethylguanine (7-etG) was lost more rapidly from hamster than from rat liver DNA, while O6-ethylguanine (O6-etG) persisted longer in hamster than in rat liver DNA. DNA ethylation was not detected in rat lung (non-target organ), while both 7-etG and O6-etG were quantitated in hamster lung (target organ) following DEN treatment. DNA ethylation in rat kidney DNA was approximately 1/10 of that in liver by 200 mg DEN/kg, and the persistence of 7-etG and O6-etG differed only slightly in these tissues. Ethylation of hamster liver DNA by DEN at doses between 20 and 200 mg/kg, as measured by 7-etG and O6-etG was proportional to the dose of carcinogen up to 160 mg/kg; at larger doses DNA ethylation sharply increased. Differences in the persistence of O6-etG between DEN-treated rats and hamsters cannot solely account for species differences in the organotropism of DEN carcinogenesis.
R A Becker; S S Lu; H Brésil; R C Shank; R Montesano
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Cancer letters     Volume:  26     ISSN:  0304-3835     ISO Abbreviation:  Cancer Lett.     Publication Date:  1985 Feb 
Date Detail:
Created Date:  1985-04-17     Completed Date:  1985-04-17     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  7600053     Medline TA:  Cancer Lett     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  17-24     Citation Subset:  IM    
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MeSH Terms
DNA / metabolism*
DNA Repair
Diethylnitrosamine / metabolism*
Liver / metabolism
Nitrosamines / metabolism*
Organ Specificity
Rats, Inbred Strains
Species Specificity
Grant Support
Reg. No./Substance:
0/Nitrosamines; 55-18-5/Diethylnitrosamine; 9007-49-2/DNA

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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