| DNA-dependent activator of IFN-regulatory factors enhances the transcription of HIV-1 through NF-κB. | |
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MedLine Citation:
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PMID: 20599623 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Pattern recognition receptors (PRRs) play a pivotal role in host innate immune responses against microbial infection. Viruses are primarily recognized by PRRs such as Toll-like receptor 3, 7, 8 and 9, and RIG-I-like receptors. Recent studies have demonstrated that DNA-dependent activator of IFN-regulatory factors (DAI) is a cytosolic sensor molecule for dsDNA, and is implicated in antiviral responses to some DNA viruses. Soon after infection, human immunodeficiency virus type-1 (HIV-1) synthesizes viral dsDNA in the cytoplasm by reverse transcriptase. In addition, an immune compromised state due to chronic HIV-1 infection results in opportunistic infection with some microbes that potentially activate DAI. However, it has not been elucidated whether DAI affects HIV-1 replication, or its possible mechanisms. Here, we showed that forced expression of DAI markedly enhanced HIV-1 replication, which was largely impaired by mutations at κB sites in HIV-1 LTR or by suppressing activation of NF-κB. Moreover, intact structure around the D3 region (174-232 aa) and two RIP homotypic interaction motifs (198-214 aa and 256-272 aa) within DAI were critical for its activity. These results suggest that activation of DAI might contribute to augment HIV-1 replication through DAI- NF-κB pathway. |
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Authors:
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Takaya Hayashi; Hironori Nishitsuji; Ayako Takamori; Atsuhiko Hasegawa; Takao Masuda; Mari Kannagi |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-06-22 |
Journal Detail:
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Title: Microbes and infection / Institut Pasteur Volume: 12 ISSN: 1769-714X ISO Abbreviation: Microbes Infect. Publication Date: 2010 Nov |
Date Detail:
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Created Date: 2010-10-25 Completed Date: 2011-01-31 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 100883508 Medline TA: Microbes Infect Country: France |
Other Details:
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Languages: eng Pagination: 937-47 Citation Subset: IM |
Copyright Information:
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Copyright © 2010 Institut Pasteur. Published by Elsevier SAS. All rights reserved. |
Affiliation:
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Department of Immunotherapeutics, Graduate School of Medicine and Dentistry, Tokyo Medical and Dental University, 1-5-45 Yushima, Bunkyo-ku, Tokyo 113-8519, Japan. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Cell Line DNA-Binding Proteins / metabolism* Gene Expression Regulation, Viral* HIV-1 / pathogenicity*, physiology Humans NF-kappa B / metabolism* Transcription, Genetic* Virus Replication |
| Chemical | |
Reg. No./Substance:
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0/DNA-Binding Proteins; 0/NF-kappa B; 0/ZBP1 protein, human |
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