| DNA damaging bystander signalling from stem cells, cancer cells and fibroblasts after Cr(VI) exposure and its dependence on telomerase. | |
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MedLine Citation:
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PMID: 19800897 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The bystander effect is a feature of low dose radiation exposure and is characterized by a signaling process from irradiated cells to non irradiated cells, which causes DNA and chromosome damage in these 'nearest neighbour' cells. Here we show that a low and short dose of Cr(VI) can induce stem cells, cancer cells and fibroblasts to chronically secrete bystander signals, which cause DNA damage in neighboring cells. The Cr(VI) induced bystander signaling depended on the telomerase status of either cell. Telomerase negative fibroblasts were able to receive DNA damaging signals from telomerase positive or negative fibroblasts or telomerase positive cancer cells. However telomerase positive fibroblasts were resistant to signals from Cr(VI) exposed telomerase positive fibroblasts or cancer cells. Human embryonic stem cells, with positive Oct4 staining as a marker of pluripotency, showed no significant increase of DNA damage from adjacent Cr and mitomycin C exposed fibroblasts whilst those cells that were negatively stained did. This selectivity of DNA damaging bystander signaling could be an important consideration in developing therapies against cancer and in the safety and effectiveness of tissue engineering and transplantation using stem cells. |
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Authors:
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Nicola Cogan; Duncan M Baird; Ryan Phillips; Lucy A Crompton; Maeve A Caldwell; Miguel A Rubio; Roger Newson; Fiona Lyng; C Patrick Case |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Mutation research Volume: 683 ISSN: 0027-5107 ISO Abbreviation: Mutat. Res. Publication Date: 2010 Jan |
Date Detail:
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Created Date: 2009-12-16 Completed Date: 2010-03-17 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0400763 Medline TA: Mutat Res Country: Netherlands |
Other Details:
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Languages: eng Pagination: 1-8 Citation Subset: IM |
Affiliation:
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Bristol Implant Research Centre, University of Bristol, Bristol, UK. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Ascorbic Acid
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pharmacology Bystander Effect / physiology* Cells, Cultured Chromium / toxicity* DNA Damage* Embryonic Stem Cells / drug effects*, metabolism Fibroblasts / drug effects*, metabolism Fluorescent Antibody Technique Histones / metabolism Humans Micronucleus Tests Signal Transduction Telomerase / metabolism* Thyroid Neoplasms / drug therapy*, metabolism Tumor Necrosis Factor-alpha / metabolism |
| Chemical | |
Reg. No./Substance:
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0/H2AFX protein, human; 0/Histones; 0/Tumor Necrosis Factor-alpha; 18540-29-9/chromium hexavalent ion; 50-81-7/Ascorbic Acid; 7440-47-3/Chromium; EC 2.7.7.49/TERT protein, human; EC 2.7.7.49/Telomerase |
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