| DNA damage responses in cell cycle G2 phase and mitosis--tracking and targeting. | |
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MedLine Citation:
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PMID: 16827124 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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BACKGROUND: In order to determine temporal responses of cell cycle populations to DNA damage, a rational combination of cell cycle analyses is critical. Moreover, the targeting of cell cycle checkpoint responses may modify the cytotoxic effect of DNA damage. MATERIALS AND METHODS: The characteristics of cell cycle populations (DNA content, cell cycle transitioning of S phase cells and size of mitotic cell fraction within the total G2/M phase population) in HeLa cells exposed to ionizing radiation were analyzed using three individual flow cytometry-based assays. The potential radiosensitization from inhibiting DNA damage responses was assessed by the colony formation assay. RESULTS: Irradiation resulted in an initial accumulation of S phase cells in G2 phase, from which the arrested cells were subsequently released to enter mitosis. Upon drug inhibition of G2 checkpoint signaling or mitotic progression, the cytotoxic effect of ionizing radiation on the HeLa cells was amplified. CONCLUSION: DNA damage-induced cell cycle responses, analyzed by selected cytometry assays and modified by specific targeting, might contribute to an understanding of how to improve radiotherapy outcome. |
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Authors:
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Anne Hansen Ree; Trond Stokke; Ase Bratland; Sebastian Patzke; Ragnhild V Nome; Sigurd Folkvord; Leonardo A Meza-Zepeda; Kjersti Flatmark; Oystein Fodstad; Yvonne Andersson |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Anticancer research Volume: 26 ISSN: 0250-7005 ISO Abbreviation: Anticancer Res. Publication Date: 2006 May-Jun |
Date Detail:
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Created Date: 2006-07-07 Completed Date: 2006-08-24 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 8102988 Medline TA: Anticancer Res Country: Greece |
Other Details:
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Languages: eng Pagination: 1909-16 Citation Subset: IM |
Affiliation:
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Department of Tumor Biology, The Norwegian Radium Hospital, 0310 Oslo, Norway. a.h.ree@medisin.uio.no |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Cell Division
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genetics,
radiation effects Cell Growth Processes / genetics, radiation effects DNA Damage / physiology* Flow Cytometry G2 Phase / genetics*, radiation effects Gene Targeting Hela Cells Humans Mitosis / genetics*, radiation effects |
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