Document Detail

DNA damage responses in cell cycle G2 phase and mitosis--tracking and targeting.
MedLine Citation:
PMID:  16827124     Owner:  NLM     Status:  MEDLINE    
BACKGROUND: In order to determine temporal responses of cell cycle populations to DNA damage, a rational combination of cell cycle analyses is critical. Moreover, the targeting of cell cycle checkpoint responses may modify the cytotoxic effect of DNA damage. MATERIALS AND METHODS: The characteristics of cell cycle populations (DNA content, cell cycle transitioning of S phase cells and size of mitotic cell fraction within the total G2/M phase population) in HeLa cells exposed to ionizing radiation were analyzed using three individual flow cytometry-based assays. The potential radiosensitization from inhibiting DNA damage responses was assessed by the colony formation assay. RESULTS: Irradiation resulted in an initial accumulation of S phase cells in G2 phase, from which the arrested cells were subsequently released to enter mitosis. Upon drug inhibition of G2 checkpoint signaling or mitotic progression, the cytotoxic effect of ionizing radiation on the HeLa cells was amplified. CONCLUSION: DNA damage-induced cell cycle responses, analyzed by selected cytometry assays and modified by specific targeting, might contribute to an understanding of how to improve radiotherapy outcome.
Anne Hansen Ree; Trond Stokke; Ase Bratland; Sebastian Patzke; Ragnhild V Nome; Sigurd Folkvord; Leonardo A Meza-Zepeda; Kjersti Flatmark; Oystein Fodstad; Yvonne Andersson
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Anticancer research     Volume:  26     ISSN:  0250-7005     ISO Abbreviation:  Anticancer Res.     Publication Date:    2006 May-Jun
Date Detail:
Created Date:  2006-07-07     Completed Date:  2006-08-24     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8102988     Medline TA:  Anticancer Res     Country:  Greece    
Other Details:
Languages:  eng     Pagination:  1909-16     Citation Subset:  IM    
Department of Tumor Biology, The Norwegian Radium Hospital, 0310 Oslo, Norway.
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MeSH Terms
Cell Division / genetics,  radiation effects
Cell Growth Processes / genetics,  radiation effects
DNA Damage / physiology*
Flow Cytometry
G2 Phase / genetics*,  radiation effects
Gene Targeting
Hela Cells
Mitosis / genetics*,  radiation effects

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