Document Detail

DNA-damage response in the basidiomycete fungus Ustilago maydis relies in a sole Chk1-like kinase.
MedLine Citation:
PMID:  19269260     Owner:  NLM     Status:  MEDLINE    
Chk1 is a protein kinase that acts as a key signal transducer within the complex network responsible of the cellular response to different DNA damages. It is a conserved element along the eukaryotic kingdom, together with a second checkpoint kinase, called Chk2/Rad53. In fact, all organisms studied so far carried at least one copy of each kind of checkpoint kinase. Since the relative contribution to the DNA-damage response of each type of kinase varies from one organism to other, the current view about the roles of Chk1 and Chk2/Rad53 during DNA-damage response is one of mutual complementation and intimate cooperation. However, in this work it is reported that Ustilago maydis - a phytopathogenic fungus exhibiting extreme resistance to UV and ionizing radiation - have a single kinase belonging to the Chk1 family but strikingly no kinases related to Chk2/Rad53 family are apparent. The U. maydis Chk1 kinase is able to respond to different classes of DNA damages and its activity is required for the cellular adaptation to such damages. As other described components of the Chk1 family of kinases, U. maydis Chk1 is phosphorylated and translocated to nucleus in response to DNA-damage signals. Interestingly subtle differences in this response depending on the kind of DNA damage are apparent, suggesting that in U. maydis the sole Chk1 kinase recapitulates the roles that in other organisms are shared by Chk1 and the Chk2/Rad53 family of protein kinases.
José Pérez-Martín
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-03-06
Journal Detail:
Title:  DNA repair     Volume:  8     ISSN:  1568-7864     ISO Abbreviation:  DNA Repair (Amst.)     Publication Date:  2009 Jun 
Date Detail:
Created Date:  2009-05-25     Completed Date:  2009-08-06     Revised Date:  2011-11-02    
Medline Journal Info:
Nlm Unique ID:  101139138     Medline TA:  DNA Repair (Amst)     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  720-31     Citation Subset:  IM    
Departamento de Biotecnología Microbiana, Centro Nacional de Biotecnología-CSIC, Campus de Cantoblanco-UAM, 28049 Madrid, Spain.
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MeSH Terms
Antibiotics, Antineoplastic / pharmacology
Cell Nucleus / drug effects,  genetics*
DNA Damage*
DNA, Fungal / physiology*
Electrophoretic Mobility Shift Assay
Enzyme Inhibitors / pharmacology
G2 Phase / drug effects
Green Fluorescent Proteins / metabolism
Hydroxyurea / pharmacology
Phleomycins / pharmacology
Phosphorylation / drug effects
Protein Kinases / physiology*
Protein Transport
Protein-Serine-Threonine Kinases / physiology
Ustilago / enzymology*,  genetics*,  growth & development
Reg. No./Substance:
0/Antibiotics, Antineoplastic; 0/DNA, Fungal; 0/Enzyme Inhibitors; 0/Phleomycins; 127-07-1/Hydroxyurea; 147336-22-9/Green Fluorescent Proteins; EC 2.7.-/Protein Kinases; EC kinase 2; EC kinase 1; EC Kinases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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