Document Detail

DNA damage and metabolic activity in the preimplantation embryo.
MedLine Citation:
PMID:  18835872     Owner:  NLM     Status:  MEDLINE    
BACKGROUND: Embryos with greater viability have a lower or 'quieter' amino acid metabolism than those which arrest. We have hypothesized this is due to non-viable embryos possessing greater cellular/molecular damage and consuming more nutrients, such as amino acids for repair processes. We have tested this proposition by measuring physical damage to DNA in bovine, porcine and human embryos at the blastocyst stage and relating the data to amino acid profiles during embryo development. METHODS: Amino acid profiles of in vitro-derived porcine and bovine blastocysts were measured by high-performance liquid chromatography and the data related retrospectively to DNA damage in each individual blastomere using a modified alkaline comet assay. Amino acid profiles of spare human embryos on Day 2-3 were related to DNA damage at the blastocyst stage. RESULTS: A positive correlation between amino acid turnover and DNA damage was apparent when each embryo was examined individually; a relationship exhibited by all three species. There was no relationship between DNA damage and embryo grade. CONCLUSIONS: Amino acid profiling of single embryos can provide a non-invasive marker of DNA damage at the blastocyst stage. The data are consistent with the quiet embryo hypothesis with viable embryos (lowest DNA damage) having the lowest amino acid turnover. Moreover, these data support the notion that metabolic profiling, in terms of amino acids, might be used to select single embryos for transfer in clinical IVF.
Roger G Sturmey; Judith A Hawkhead; E Ann Barker; Henry J Leese
Publication Detail:
Type:  Journal Article     Date:  2008-10-03
Journal Detail:
Title:  Human reproduction (Oxford, England)     Volume:  24     ISSN:  1460-2350     ISO Abbreviation:  Hum. Reprod.     Publication Date:  2009 Jan 
Date Detail:
Created Date:  2008-12-09     Completed Date:  2009-02-06     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8701199     Medline TA:  Hum Reprod     Country:  England    
Other Details:
Languages:  eng     Pagination:  81-91     Citation Subset:  IM    
Biology Department (Area 3), University of York, York YO10 5YW, UK.
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MeSH Terms
Amino Acids / metabolism*
Blastocyst / metabolism*
Blastomeres / metabolism
Chromatography, High Pressure Liquid
Comet Assay
Culture Media / analysis
DNA Damage*
Embryonic Development
Swine / embryology
Reg. No./Substance:
0/Amino Acids; 0/Culture Media

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