| [DNA damage in lymphocytes after irradiation with 211At and 188Re.]. | |
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MedLine Citation:
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PMID: 19847357 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
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Aim: Ionising radiation produces many types of DNA lesions of different complexity. High linear energy transfer (LET) types of radiation are biological more effective than low LET radiation. In the present work we applied the single cell gel electrophoreses (comet assay) to study the induction of initial DNA damage, efficiency of repair and residual DNA damage in lymphocytes after treatment with 211At and 188Re. Methods: Peripheral blood mononuclear cells (PBMC) were isolated from heparinized blood of healthy donors and irradiated with 211At and 188Re at different doses. The comet assay was performed under alkaline and neutral conditions in order to detect the initial DNA damage and its repair. The measure of damage was % tail DNA (percentage of DNA in the tail). Results: After treatment of cells with 188Re the initial DNA damage (% tail DNA) detected with the alkaline comet assay was higher than the damage measured for 211At. The neutral comet assay estimated higher tail intensities for 211At in contrast to 188Re. Compared with the complete repair (10%) after irradiation with 188Re, the radiotoxicity of alpha particles indicated reduced rejoining of DNA strand breaks (60-80% residual damage). Rejoining of DNA damage measured by the neutral comet method detected about 70% unrepaired strand breaks for 211At and 188Re. Conclusions: There are major differences between the repair of strand breaks caused by 188Re and 211At detected by the alkaline comet assay. The DNA-damage induced by the high LET Emitter 211At remains nearly unrepaired detected by both alkaline and neutral comet assay. Represented data following irradiation of lymphocytes with alpha and beta particles demonstrated higher biological effectiveness of 211At by factors of 2.0-2.5. |
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Authors:
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R Runge; M Wendisch; G Wunderlich; R Freudenberg; J Kotzerke |
Publication Detail:
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Type: English Abstract; Journal Article Date: 2009-10-22 |
Journal Detail:
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Title: Nuklearmedizin. Nuclear medicine Volume: 48 ISSN: 0029-5566 ISO Abbreviation: Nuklearmedizin Publication Date: 2009 |
Date Detail:
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Created Date: 2009-12-07 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 7609387 Medline TA: Nuklearmedizin Country: Germany |
Other Details:
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Languages: ger Pagination: 221-6 Citation Subset: IM |
Affiliation:
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Roswitha Runge, Klinik und Poliklinik für Nuklearmedizin, Universitätsklinikum Dresden, Fetscherstr. 74, 01307 Dresden, Tel. 03 51/458 54 81, Fax 03 51/458 53 47, E-Mail: roswitha.runge@uniklinikum-dresden.de. |
Vernacular Title:
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DNA-Schäden von Lymphozyten nach Bestrahlung mit 211At und 188Re - Quantifizierung mit dem alkalischen und neutralen Komet-Assay. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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