Document Detail


DNA-based rhesus typing: simultaneous determination of RHC and RHD status using the polymerase chain reaction.
MedLine Citation:
PMID:  8740009     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We describe a PCR-based method of performing RHD and C/c typing in a single reaction. The method is based on an earlier observation of a polymorphism in intron 2 of both genes which, in addition to detecting the RHD deletion responsible for most known D-negative phenotypes, is also associated with C/c serological type. Using this assay, we typed 105 unrelated individuals from at least four different population groups and compared the results to those obtained using conventional serological testing of red cells. An absolute correlation, with no exceptions, was seen. We also showed that the method has potential in the antenatal determination of RH type, as it was possible to type fetal trophoblasts recovered from the endocervical canal at 9 weeks pregnancy.
Authors:
M Poulter; T J Kemp; B Carritt
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Vox sanguinis     Volume:  70     ISSN:  0042-9007     ISO Abbreviation:  Vox Sang.     Publication Date:  1996  
Date Detail:
Created Date:  1996-12-05     Completed Date:  1996-12-05     Revised Date:  2008-08-29    
Medline Journal Info:
Nlm Unique ID:  0413606     Medline TA:  Vox Sang     Country:  SWITZERLAND    
Other Details:
Languages:  eng     Pagination:  164-8     Citation Subset:  IM    
Affiliation:
MRC Human Biochemical Genetics Unit, University College London, UK.
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MeSH Terms
Descriptor/Qualifier:
Blood Grouping and Crossmatching / methods*
Blotting, Southern
DNA / chemistry*
Deoxyribonucleases, Type II Site-Specific / metabolism
Electrophoresis, Agar Gel
Female
Gene Deletion
Humans
Introns
Nucleic Acid Hybridization
Phenotype
Polymerase Chain Reaction / methods*
Polymorphism, Restriction Fragment Length
Pregnancy
Rh-Hr Blood-Group System / genetics*
Chemical
Reg. No./Substance:
0/Rh-Hr Blood-Group System; 0/Rho(D) antigen; 9007-49-2/DNA; EC 3.1.21.4/CTGCAG-specific type II deoxyribonucleases; EC 3.1.21.4/Deoxyribonucleases, Type II Site-Specific

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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