Document Detail


The DNA-based human karyotype.
MedLine Citation:
PMID:  6205836     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Image cytometry and computer analysis are used to determine the relative DNA content and the DNA-based centromeric index of the 24 chromosomes of the human karyotype. A two-step procedure is used. Chromosomes of cells in metaphase first are stained with quinacrine and identified visually by their fluorescent Q-band patterns. They then are stained for DNA using gallocyanin-chrome alum. The chromosome images are scanned and recorded as digital values of optical density by an CYDAC image cytometric microscope system, CYDAC. The digital images are processed by computer to measure for each chromosome the relative DNA stain contents of the whole chromosome and of the p and q arms and the DNA-based centromeric index. About ten cells are analyzed for each of the donors, who are phenotypically normal men and women. The chromosome measurements are pooled by chromosome type for each donor and are compared among donors. The means of the chromosome measurements give the DNA-based human karyotype. Analysis of the DNA-based data shows that some chromosomes or portions of chromosomes vary significantly among donors. These variants do not correlate with detectable morphologic polymorphisms, such as Q- or C-band variants; thus they represent new and otherwise undetectable chromosome polymorphisms whose genetic basis and clinical significance are yet to be determined.
Authors:
B H Mayall; A V Carrano; D H Moore; L K Ashworth; D E Bennett; M L Mendelsohn
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Cytometry     Volume:  5     ISSN:  0196-4763     ISO Abbreviation:  Cytometry     Publication Date:  1984 Jul 
Date Detail:
Created Date:  1984-10-01     Completed Date:  1984-10-01     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  8102328     Medline TA:  Cytometry     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  376-85     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Adult
Cells, Cultured
Centromere / analysis*
Chromosome Banding
Chromosomes / analysis*
Chromosomes, Human / analysis*
DNA / analysis*
Flow Cytometry / methods
Humans
Karyotyping*
Metaphase
Staining and Labeling
Grant Support
ID/Acronym/Agency:
GM-20291/GM/NIGMS NIH HHS
Chemical
Reg. No./Substance:
9007-49-2/DNA

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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