Document Detail


The DNA of bacteriophage 643: isolation and properties of the DNA of a bacteriophage infecting lactic Streptococci.
MedLine Citation:
PMID:  6324460     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The DNA extracted from bacteriophage 643, which infects group N strains of Streptococci, could be separated into four components by electrophoresis on agarose gels. Electron microscopy established the predominant form of the DNA to be a relaxed, circular molecule of molecular weight 14.9 X 10(6). The four species observed on electrophoresis are believed to be a circular monomeric form, a linear form derived from it, a circular dimeric form, and the corresponding linear dimer. Two DNA components were demonstrated by sedimentation velocity in the analytical ultracentrifuge and by zone sedimentation in sucrose gradients. These corresponded to a circular monomeric form and a circular dimeric form; no evidence for supercoiled forms was found by sedimentation in either neutral or denaturing solvents. Bacteriophage 643 DNA was sensitive to cleavage by single-strand-specific nuclease S1 and could be labeled in vitro by the PolI-catalyzed incorporation of [alpha-32P]dATP into the molecule under conditions that did not permit nick translation, suggesting that the circular duplex molecule is interrupted by a single-stranded gap.
Authors:
D J Lyttle; G B Petersen
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Virology     Volume:  133     ISSN:  0042-6822     ISO Abbreviation:  Virology     Publication Date:  1984 Mar 
Date Detail:
Created Date:  1984-05-18     Completed Date:  1984-05-18     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0110674     Medline TA:  Virology     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  403-15     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Bacteriophages / genetics*
Base Sequence
DNA Restriction Enzymes
DNA, Bacterial / genetics,  isolation & purification
DNA, Viral / genetics,  isolation & purification*
Lactococcus lactis / genetics*
Microscopy, Electron
Molecular Weight
Chemical
Reg. No./Substance:
0/DNA, Bacterial; 0/DNA, Viral; EC 3.1.21.-/DNA Restriction Enzymes

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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